Non-small cell lung cancer (NSCLC) constitutes nearly 85% of all cases of lung cancer. Drug resistance, dose-limiting toxicity, and metastasis in NSCLC eventually reduce the efficacy of chemotherapeutics. In this study, we have shown that the methanol-ethyl acetate partitioned fraction from Magnolia grandiflora L. seeds (MEM) exhibit potential anti-cancer activities against NSCLC H1975 cells in vivo and in vitro. MEM significantly inhibited the proliferation of H1975 cells in a concentration- and time-dependent manner. Further, MEM exhibited potent anti-tumor efficacy and low toxicity in nude mice bearing H1975 tumors. Our study also showed that MEM could induce cellular apoptosis in H1975 cells by down-regulating the protein expression levels of Akt and p-Akt-473, and by increasing the ratio of Bax/Bcl-2. Also, MEM significantly inhibited metastasis-related cell invasion and migration of H1975 cells, which associated with the down-regulation of HIF-1α, MMP-2, and MMP-9 protein expression levels. Thus, our data shows that MEM may be an effective fraction of M. grandiflora in NSCLC treatment.

非小细胞肺癌（NSCLC）占所有肺癌病例的近85％。NSCLC中的耐药性，剂量限制性毒性和转移最终会降低化疗药物的功效。在这项研究中，我们已经显示了木兰种子（MEM）的甲醇-乙酸乙酯分配馏分在体内和体外表现出对NSCLC H1975细胞的潜在抗癌活性。。MEM以浓度和时间依赖性方式显着抑制H1975细胞的增殖。此外，MEM在带有H1975肿瘤的裸鼠中显示出有效的抗肿瘤功效和低毒性。我们的研究还表明，MEM可以通过下调Akt和p-Akt-473的蛋白表达水平以及增加Bax / Bcl-2的比例来诱导H1975细胞凋亡。同样，MEM显着抑制了H1975细胞与转移相关的细胞侵袭和迁移，这与HIF-1α，MMP-2和MMP-9蛋白表达水平的下调有关。因此，我们的数据表明，在NSCLC治疗中，MEM可能是大花曼陀罗的有效成分。