Melanocytes are melanin (pigment)‐producing cells that are primarily located in the stratum basale of the epidermis comprising 5% of the epidermal cells in normal human skin (Haass & Herlyn, 2005). Melanocytes show high expression of CD117 (c‐Kit)(Cario & Taieb, 2019) and can be visualized in skin sections by specific immunohistochemical staining for this marker (Figure 1a). To date, human melanocyte monocultures can be obtained from epidermal cell suspensions by suppressing growth of contaminating keratinocytes and fibroblasts (Cario & Taieb, 2019; Godwin et al., 2014; Hsu, Li, & Herlyn, 2005; Zhang et al., 2018). Although this procedure ultimately provides pure melanocyte cell populations, it is quite time consuming (it takes several weeks of in vitro culture) and there is a potential risk that melanocytes undergo phenotypical and functional changes during culturing.

中文翻译：

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从新鲜制备的表皮细胞悬液中即时分离高度纯化的人黑素细胞。

黑色素细胞是产生黑色素（色素）的细胞，主要位于表皮基底层，占正常人皮肤中表皮细胞的5％（Haass＆Herlyn，2005）。黑色素细胞显示高表达的CD117（c‐Kit）（Cario＆Taieb，2019），并且可以通过对该标记物进行特异性免疫组织化学染色在皮肤切片中观察到（图1a）。迄今为止，通过抑制污染性角质形成细胞和成纤维细胞的生长，可以从表皮细胞悬浮液中获得人黑素细胞的单一培养物（Cario＆Taieb，2019; Godwin等，2014; Hsu，Li，＆Herlyn，2005; Zhang等，2018）。 ）。尽管此程序最终可以提供纯净的黑素细胞群，但仍非常耗时（在体外需要数周的时间 培养过程中），并且存在黑素细胞在培养过程中发生表型和功能变化的潜在风险。