The degree of resin swelling in a particular solvent system is one of the critical parameters for solid‐phase peptide synthesis (SPPS) and for solid‐phase synthesis in general. Methods used for measuring the degree of resin swelling include microscopy‐based and volumetry‐based methods. This study describes and compares the use of both methods for a number of commercially available resins commonly used in SPPS, with a range of solvents, which have been identified in the literature as ‘greener’ than DCM, DMF and NMP. The results were analysed by statistical methods, and a significant correlation between the two distinct methods has been demonstrated for the first time. The results will likely be used, in conjunction with other literature methods, to help in choosing both the resin and solvent system for greener SPPS, as well as for continuous flow SPPS, which is of growing importance.

中文翻译：

---

评估在一系列潜在的SPPS绿色溶剂中树脂溶胀的基于显微镜和体积法的相关性。

特定溶剂系统中树脂的溶胀度是固相肽合成（SPPS）和一般固相合成的关键参数之一。用于测量树脂溶胀度的方法包括基于显微镜的方法和基于体积法的方法。这项研究描述并比较了两种方法在SPPS中通常使用的许多市售树脂以及一系列溶剂的使用，这些溶剂在文献中已被确定为比DCM，DMF和NMP更为“绿色”。通过统计方法对结果进行了分析，并且首次证明了两种不同方法之间的显着相关性。该结果可能会与其他文献方法结合使用，以帮助选择树脂和溶剂体系，以获得更绿色的SPPS，