Whole-genome next-generation sequencing was used to investigate the local evolution of viral haemorrhagic septicaemia virus, a serious pathogen affecting economically important fish such as rainbow trout and turbot in Europe and olive flounder in Asia. Sequence analysis showed that all isolates were genotype IVa, but could be classified further into four subgroups (K1-K4). In addition, genomic regions encompassing the nucleoprotein, phosphoprotein, matrix protein and non-virion protein genes, as well as the seven non-coding regions, were relatively conserved, whereas glycoprotein and RNA-dependent RNA polymerase genes were variable in the coding region. Taken together, the data demonstrate that whole-genome next-generation sequencing may be useful for future surveillance, prevention and control strategies against viral haemorrhagic septicaemia.

中文翻译：

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韩国病毒性败血病病毒的全基因组下一代测序和系统发育特征。

全基因组下一代测序用于研究病毒性出血性败血病病毒的局部进化，该病毒是一种严重的病原体，会影响经济上重要的鱼类，例如欧洲的虹鳟鱼和大菱turbo以及亚洲的橄榄比目鱼。序列分析表明，所有分离株均为基因型IVa，但可以进一步分为四个亚组（K1-K4）。此外，包含核蛋白，磷蛋白，基质蛋白和非病毒体蛋白基因的基因组区域以及七个非编码区相对保守，而糖蛋白和依赖RNA的RNA聚合酶基因在编码区可变。综上所述，数据表明，全基因组下一代测序可能对将来针对病毒性败血病的监测，预防和控制策略很有用。