Plant Cell, Tissue and Organ Culture ( IF 3 ) Pub Date : 2020-02-18 , DOI: 10.1007/s11240-020-01793-7 Craig H. Carlson , Todd P. West
Key message
Approach for reducing the growth-inhibiting effects of endogenous phenolic compounds in katsuratree (Cercidiphyllum japonicum).
Abstract
An approach for reducing the growth-inhibiting effects of endogenous phenolic compounds in katsuratree (Cercidiphyllum japonicum Sieb. & Zucc.) was developed to assist with clonal micropropagation of the species. A quantitative phenolic profile of Cercidiphyllum spp. was undertaken to provide further evidence of metabolic diversity within the genus. In order to mitigate the growth-inhibiting effects of preformed and induced polyphenols in katsura microshoot cultures, a range of sequestering treatments and carbon sources were compared. Cultures were compared by presoaking nodal explants in aqueous solutions for 12 or 24 h as well as incorporating sequestering agents into initiation media. The carbon source treatments, sucrose and d-(+)-glucose, were compared at concentrations of 0%, 1.5%, 3%, and 5% (w/v), and 1:1 (w/v) mixtures of d-(+)-glucose and sucrose over 4 weeks on a bulked, half-sib C. japonicum family. Eight major phenolic compounds were identified in varying concentrations and presence within and among species. Presoaking solutions significantly outperformed all other treatments for shoot growth traits. Incorporation of l-ascorbic acid at 40 μM showed similar performance. The carbon source d-(+)-glucose at 3% (w/v) was determined to be the most effective treatment for improving microshoot growth.
中文翻译:
胜树(Cercidiphyllum spp。)微芽培养物中多酚诱导的顽固性减轻
关键信息
降低内生酚类化合物在葛根树中的生长抑制作用的方法。
抽象
为了减少该物种的克隆微繁,开发了一种减少内生酚类化合物在葛缕子中的生长抑制作用的方法(Cercidiphyllum japonicum Sieb。&Zucc。)。cerceridiphyllum spp的定量酚谱。旨在提供该属内代谢多样性的进一步证据。为了减轻在katsura microshoot培养中预先形成的和诱导的多酚的生长抑制作用,比较了一系列螯合处理和碳源。通过将节点外植体在水溶液中预浸泡12或24小时,以及将螯合剂掺入起始培养基中来比较培养物。碳源处理,蔗糖和d-(+)-葡萄糖的浓度分别为d -(+)-葡萄糖和蔗糖的0%,1.5%,3%和5%(w / v)和1:1(w / v)混合物的浓度在一个庞大的半同胞日本血吸虫家族中度过了四个星期。确定了八种主要的酚类化合物,它们在物种内部和物种之间的浓度和存在程度均不同。预浸液的效果明显优于其他所有处理方法。以40μM掺入1-抗坏血酸表现出相似的性能。确定3%(w / v)的碳源d -(+)-葡萄糖是改善微枝生长的最有效方法。