当前位置: X-MOL 学术Plant Cell Tiss. Organ Cult. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Influence of Vitrofural ® on sugarcane micropropagation using temporary immersion system
Plant Cell, Tissue and Organ Culture ( IF 3 ) Pub Date : 2020-02-21 , DOI: 10.1007/s11240-020-01800-x
Alina Martínez Rivero , Marco A. Ramírez-Mosqueda , Osbel Mosqueda Frómeta , Maritza M. Escalona Morgado , Maribel Rivas Paneca , Rene C. Rodríguez Escriba , Marcos A. Daquinta Gradaille , Jericó J. Bello-Bello

Abstract

Sugarcane cultivation is of great economic and social importance worldwide. In vitro cloning using temporary immersion systems is an option to produce vitroplants of high genetic and phytosanitary quality to establish stock seedbeds. However, the sterilization of culture media through autoclaving increases production costs and limits the efficiency of temporary immersion systems. For that reason, the objective of this study was to evaluate the morphological and physiological changes caused by Vitrofural® in sugarcane plants under temporary immersion. We compared two types of culture media sterilization: chemical sterilization using Vitrofural® and autoclaving using Temporary Immersion Bioreactors. After four weeks of cultivation, we evaluated morphological (number of shoots, shoot length, and number of leaves per shoot) and physiological indicators (soluble and bound phenolics, and phenolics excreted to the culture medium). As well as chlorophyll-a, -b and total chlorophyll content. The results show that when Vitrofural® was added to culture medium, a higher number of shoots per explant, length of the shoots and number of leaves were observed in comparison with the shoots obtained in autoclaved sterilized medium. Furthermore, a reduced the amount of phenolics compounds were found in Vitrofural® treatent .In addition, the use of the Vitrofural® increased the synthesis of photosynthetic pigments respect to autoclaving. In conclusion, Vitrofural® has a positive effect in morphological and physiological of sugarcane cultured in vitro and is a low-cost alternative for the commercial micropropagation of sugarcane.

Key message

Vitrofural® produced a positive effect on the growth, content of photosynthetic pigments and compounds phenols during in vitro culture of sugar cane.



中文翻译:

Vitrofural®对使用临时浸没系统的甘蔗微繁殖的影响

摘要

甘蔗种植在世界范围内具有重要的经济和社会意义。使用临时浸没系统进行体外克隆是生产具有高遗传和植物检疫质量的体外植物以建立苗床的一种选择。然而,通过高压灭菌对培养基进行灭菌增加了生产成本,并限制了临时浸没系统的效率。因此,这项研究的目的是评估造成Vitrofural形态和生理变化®下临时浸入甘蔗植株。我们比较了两种类型的培养基灭菌:用Vitrofural化学灭菌®使用临时浸入式生物反应器进行高压灭菌。培养四周后,我们评估了形态学(枝条数,枝条长度和每枝枝叶数)和生理指标(可溶性和结合酚类,以及从培养基中排泄的酚类)。以及叶绿素a,-b和总叶绿素含量。结果表明,当Vitrofural ®加入到培养基中,较高数量的每个外植芽,与在高压灭菌灭菌的培养基中获得的枝条比较观察枝条和叶的数目的长度。此外,减小的酚类化合物的量在Vitrofural发现® treatent。此外,使用该Vitrofural的®就高压灭菌而言,增加了光合色素的合成。总之,Vitrofural ®具有形态学甘蔗体外培养的积极作用和生理和是一个低成本的可替代为甘蔗的商业微量繁殖。

关键信息

Vitrofural ®产生体外甘蔗的培养过程中的生长,光合色素和化合物酚的含量具有积极效果。

更新日期:2020-04-22
down
wechat
bug