AAL toxin, the major virulence factor of Alternaria alternata f. sp. lycopersici, is recognized to cause necrotic cell death in plants. Glutathione (GSH) is a noteworthy participant in plant defence. However, how GSH is involved in regulating the AAL treated cell death is yet to be explored. Here, Arabidopsis thaliana Col-0, and previously developed transgenic line AtECS1, were exogenously treated with AAL toxin and a proteomic profile (ProteomeXchange accession: PXD017124) was obtained by nano LC–MS/MS analysis. Few salicylic acid (SA) and ethylene (ET) responsive proteins, along with others were identified. Selected SA-responsive genes were noted to be up regulated in AAL treated AtECS1 compared to Col-0 by quantitative real time-PCR (qRT-PCR), beside the up regulation of ascorbate peroxidase 1 (APX1) and chaperone like heat shock protein (HSP), together with myrosinase. Interestingly, ET biosynthetic and signaling marker genes were down regulated in AAL treated AtECS1 compared to Col-0. Augmentation of SA content and proteins regulated by it, while, reduction of endogenous 1-aminocyclopropane-1-carboxylate (ACC) content and ET-related proteins was significant in AAL treated AtECS1 compared to Col-0. Collectively, these findings suggested that under necrotrophic attack as mimicked here by AAL treatment, GSH may be involved in resistance primarily by SA-mediated ET suppression in addition to various stress responsive molecules.

AAL毒素，Alternaria alternata f的主要毒力因子。sp。lycopersici被认为会导致植物坏死细胞死亡。谷胱甘肽（GSH）是植物防御的重要参与者。但是，如何研究GSH参与调节AAL处理的细胞死亡。在这里，拟南芥Col-0和先前开发的转基因品系AtECS1用AAL毒素进行外源处理，并通过纳米LC-MS / MS分析获得了蛋白质组学特征（ProteomeXchange保藏号：PXD017124）。几乎没有发现水杨酸（SA）和乙烯（ET）响应蛋白。注意到选定的SA反应基因在AAL处理的AtECS1中被上调通过实时定量PCR（qRT-PCR）与Col-0相比，除了抗坏血酸过氧化物酶1（APX1）和伴侣蛋白样热休克蛋白（HSP）的上调外，还有黑芥子酶。有趣的是，与Col-0相比，AAL处理的AtECS1中的ET生物合成和信号标记基因被下调。在经AAL处理的AtECS1中，SA含量和受其调节的蛋白质的含量增加，而内源性1-氨基环丙烷-1-羧酸盐（ACC）含量和与ET相关的蛋白质的减少是显着的相比于Col-0。总体而言，这些发现表明，在通过AAL处理模仿此处的坏死性攻击下，除各种应激反应分子外，GSH可能还主要通过SA介导的ET抑制参与了耐药。