当前位置: X-MOL 学术Plant Cell Tiss. Organ Cult. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Colchicine in vitro tetraploid induction of Populus hopeiensis from leaf blades
Plant Cell, Tissue and Organ Culture ( IF 3 ) Pub Date : 2020-02-18 , DOI: 10.1007/s11240-020-01790-w
Jian Wu , Yaru Sang , Qing Zhou , Pingdong Zhang

A protocol for tetraploid in vitro induction of diploid Populus hopeiensis leaf blades by colchicine was established. Leaf blades were pre-cultured in MS basal medium containing 1.78 μM 6-BA, 0.07 μM TDZ, and 0.53 μM IAA for 5, 6, and 7 days, and then transferred to a liquid MS medium supplemented with colchicine at concentrations of 50, 75, and 100 μM for 48, 72, and 96 h. We found that pre-culture duration and exposure time had moderate effects on the tetraploid induction rate, and colchicine concentration had highly significant effects on tetraploid induction rate. The highest tetraploid induction rate (21.2%) was observed when leaf blades were pre-cultured for 7 days and cultured for 96 h in liquid MS with 100 μM colchicine. Flow cytometry analysis was used to detect putative tetraploids from the regenerated plants, and chromosome number counting was employed to confirm the polyploidy level. A total of 54 tetraploids were obtained. Significant differences in stomatal length, stomatal width, and density of leaf stomata between diploid and tetraploid plants were observed, suggesting that tetraploid plants present modified morphological characteristics.



中文翻译:

秋水仙碱从叶片中诱导希望杨的四倍体诱导

四倍体离体希望二倍体杨的协议。建立秋水仙碱的叶片。将叶片在含有1.78μM6-BA,0.07μMTDZ和0.53μMIAA的MS基础培养基中预培养5天,6天和7天,然后将其转移至补充有秋水仙碱浓度为50, 75和100μM,持续48、72和96小时。我们发现,培养前的持续时间和暴露时间对四倍体诱导率有中等程度的影响,秋水仙碱浓度对四倍体诱导率有非常显着的影响。当叶片预培养7天并在含有100μM秋水仙碱的液体MS中培养96 h时,观察到最高的四倍体诱导率(21.2%)。流式细胞仪分析用于检测再生植物的推定四倍体,并利用染色体数目计数来确认多倍体水平。总共获得54个四倍体。在二倍体和四倍体植物之间观察到气孔长度,气孔宽度和叶片气孔密度的显着差异,表明四倍体植物呈现出改良的形态特征。

更新日期:2020-04-22
down
wechat
bug