In vitro cultures give the opportunity to perform the phytochemical studies on the protected species without harvesting the plant material from the natural environment. Shoots of Eryngium alpinum L. were multiplied on Murashige and Skoog (MS) medium in various systems, namely on the solid media and in two liquid cultures—stationary and agitated, as well as via regeneration from callus. The biomass increments were closely correlated with the number of shoots arising from one explant, which was connected with the supplementation of the culture media with the studied plant growth regulators. The methanolic extracts from shoots grown in the tested systems were subjected to phenolic acids and flavonoids qualitative and quantitative analysis. Biomass from in vitro shoot cultures accumulated from 19.59 to 32.95 times more phenolic acids [the total content ranged from 272.52 to 458.38 mg/100 g dry weight (DW)] and from 3.02 to 4.43 times more flavonoids (the total content ranged from 100.03 to 146.98 mg/100 g DW), depending on the culture system, than the extracts from basal leaves from the intact plant (13.91 and 33.16 mg/100 g DW, respectively). The phenolics present in shoot cultures include seven phenolic acids—3,4-dihydroxyphenylacetic, caftaric, caffeic, neochlorogenic, chlorogenic, isochlorogenic, and rosmarinic acids, and three flavonoids—isoquercetin, quercitrin and robinin. The best system for shoot proliferation resulting in the highest biomass growth and phenolic acids and flavonoids accumulation was solid culture on MS medium with BAP, IAA, and GA₃ (each 1.0 mg/l). The aim of this work was to check the effect of various culture systems (stationary and agitated, on solidified and in liquid media) on the production of phenolic compounds in E. alpinum shoots cultured in vitro.

体外培养提供了对受保护物种进行植物化学研究的机会，而无需从自然环境中收获植物材料。刺芹的芽L.在各种系统的Murashige和Skoog（MS）培养基上繁殖，即在固体培养基上和在两种液体培养物中（固定和搅动）以及通过愈伤组织的再生繁殖。生物量的增加与一种外植体产生的芽数密切相关，这与所研究的植物生长调节剂对培养基的补充有关。对在测试系统中生长的新芽中的甲醇提取物进行酚酸和类黄酮的定性和定量分析。体外芽培养物中的生物质比酚酸多积累了19.59至32.95倍[总含量范围为272.52至458.38 mg / 100 g干重（DW）]，而类黄酮的累积量为3.02至4.43倍（总含量范围为100.03至100。 146.98 mg / 100 g DW），具体取决于培养系统，比完整植物基叶的提取物要多（分别为13.91和33.16 mg / 100 g DW）。芽培养物中存在的酚类化合物包括七种酚酸（3,4-二羟基苯基乙酸，咖啡酸，咖啡酸，新绿原酸，绿原酸，异绿原酸和迷迭香酸）和三种黄酮类化合物（异槲皮素，槲皮苷和罗宾宁）。在BAP，IAA和GA的MS培养基上进行固体培养，从而获得最佳的芽增殖系统，从而实现最高的生物量增长以及酚酸和类黄酮的积累。槲皮素和知更鸟。在BAP，IAA和GA的MS培养基上进行固体培养，从而获得最佳的芽增殖系统，从而实现最高的生物量增长以及酚酸和类黄酮的积累。槲皮素和知更鸟。在BAP，IAA和GA的MS培养基上进行固体培养，从而获得最佳的芽增殖系统，从而实现最高的生物量增长以及酚酸和类黄酮的积累。₃（每个1.0 mg / l）。这项工作的目的是检查各种培养系统（固定的和搅拌的，固化的和在液体介质中的培养系统）对体外培养的大肠大肠杆菌芽中酚类化合物产生酚的影响。