An efficient plant regeneration protocol was developed for Basilicum polystachyon (L.) Moench using shoot tip from in vitro germinated plant. Both shoot multiplication and root induction were initiated from shoot tip explants in Murashige and Skoog’s (MS) basal medium supplemented with N⁶-benzylaminopurine (BAP) and 6-furfurylaminopurine (Kin) combination with 1-naphthaleneacetic acid (NAA) and without any plant growth regulator. Among the different concentrations and combinations of growth regulators, the highest number of shoots per explants was induced on 13.32 μM BAP with 0.53 μM NAA. It was also found that the multiplication of shoots along with roots induced in MS medium without any plant growth regulators. The in vitro grown plants were successfully hardened and acclimatized in the field with a 99% survival rate. The results obtained from HPLC analysis established the presence of a significant amount of endogenous auxin, viz. indole-3-acetic acid acid and indole-3-butyric acid in the shoot and root tips of B. polystachyon. This is the first report of a successful multiplication of B. polystachyon in absence of plant growth regulators and the presence of an abundant quantity of endogenous auxin in root and shoot tips using Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) coupled with ultraviolet–visible (UV–Vis) detector.

使用来自萌发的植物的芽尖，为Basilicum polystachyon（L.）Moench开发了一种有效的植物再生方案。在补充有N ^6的Murashige和Skoog（MS）基础培养基中，从芽尖外植体开始芽繁殖和根诱导。-苄基氨基嘌呤（BAP）和6-糠基氨基嘌呤（Kin）与1-萘乙酸（NAA）的组合，并且没有任何植物生长调节剂。在不同浓度和生长调节剂的组合中，在13.32μMBAP和0.53μMNAA上诱导了每个外植体的最高芽数。还发现在没有任何植物生长调节剂的MS培养基中诱导的芽和根的繁殖。体外生长的植物在田间成功地变硬并适应环境，成活率达99％。通过HPLC分析获得的结果证实存在大量的内源性生长素。B. polystachyon芽和根尖中的吲哚-3-乙酸和吲哚-3-丁酸。这是成功相乘的第一份报告。B.使用反相高效液相色谱（RP-HPLC）结合紫外-可见（UV-Vis）检测器，在没有植物生长调节剂且根和茎尖中存在大量内源性生长素的情况下，多水苏属。