当前位置: X-MOL 学术Mycol. Progress › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Optical study of laser biospeckle activity in leaves of Jatropha curcas L.: a non-invasive and indirect assessment of foliar endophyte colonization
Mycological Progress ( IF 2.4 ) Pub Date : 2020-03-11 , DOI: 10.1007/s11557-020-01563-x
Maria Fernanda D’Jonsiles , Gustavo Ernesto Galizzi , Andres Ezequiel Dolinko , María Victoria Novas , Esteban Ceriani Nakamurakare , Cecilia Cristina Carmarán

Currently, the detection of endophytic fungi is mostly determined by invasive methods, including direct isolation of fungal organisms from plant tissue in growth media, molecular detection of endophytic fungi DNA from plant material by PCR, or evaluation using microscopy techniques. In this work, we explore the potential of laser biospeckle activity (LBSA) for the detection of endophytic colonization of leaves of a promising energy crop, Jatropha curcas L. We compared the laser biospeckle activity of endophyte infected and uninfected J. curcas leaves. The differences between blade and veins (including midrib) of the studied leaves were validated and growth parameters of the studied plants were also analyzed using the normalized weighted generalized differences coefficient (nWGD), which characterizes a particular laser bioactivity behavior. The obtained results showed a relationship between the endophytic burden of leaves and the LBSA, suggesting that LBSA is a useful tool to indirectly detect endophytic colonization in situ. Also, the increased water movements inside leaves promoted by endophytic colonization could be explained by the obtained data.

中文翻译:

麻疯树叶片中激光生物散斑活性的光学研究:叶内生菌定植的非侵入性和间接评估

当前,内生真菌的检测主要通过侵入性方法来确定,包括从生长培养基中的植物组织中直接分离真菌生物,通过PCR从植物材料中分子检测内生真菌DNA或使用显微镜技术进行评估。在这项工作中,我们将探讨激光biospeckle活动(LBSA)用于检测看好能源作物的叶片内生定居的潜力,麻疯树属我们比较了内生真菌感染的激光biospeckle活动和未感染麻疯树树叶。验证了研究叶片的叶片和叶脉(包括中脉)之间的差异,并使用归一化加权广义差异系数(nWGD)分析了研究植物的生长参数,该系数表征了特定的激光生物活性行为。所得结果表明叶片的内生负荷与LBSA之间存在关系,这表明LBSA是间接检测原位内生菌落的有用工具。另外,通过内生菌落定植促进了叶片内部水分运动的增加。
更新日期:2020-03-11
down
wechat
bug