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High prevalence of Cryptosporidium infection caused by C. scrofarum and C. suis among pigs in Thailand.
Parasitology International ( IF 1.9 ) Pub Date : 2020-04-09 , DOI: 10.1016/j.parint.2020.102122
Umaporn Thathaisong 1 , Suradej Siripattanapipong 2 , Tawin Inpankaew 3 , Saovanee Leelayoova 4 , Mathirut Mungthin 4
Affiliation  

Cryptosporidium spp. is an important intestinal protozoan causing diarrhea among both healthy and immunocompromised patients especially those with HIV/AIDS. Cryptosporidium spp. can be transmitted via foodborne, waterborne and person-to-person routes. In addition, several Cryptosporidium species are zoonotic. This study aimed to determine the prevalence of Cryptosporidium infection among pigs raised in both smallholder (<50 heads/farm) and large scale farms (50–500 heads/farm) in Chonburi Province, eastern Thailand using nested PCR amplifying the small subunit of the ribosomal RNA (SSU-rRNA) gene. DNA sequencing was also performed to identify the species of Cryptosporidium. A total of 245 fecal samples were collected from 11 pig farms. The overall prevalence of Cryptosporidium infection was 20.8% (51/245) which were found in both smallholder and small large scale pig farms. The prevalence of Cryptosporidium infection among pigs aged ≤6 months was significantly higher than those aged >6 months (p < .001). Among 51 Cryptosporidium positive samples, Cryptosporidium scrofarum (42/51, 82.4%) and Cryptosporidium suis (9/51, 17.6%) were identified. The prevalence of C. scrofarum infection observed among pigs aged ≤6 months was significantly higher when compared with those aged >6 months (20.7% and 2.1%, respectively, p < .001). The high prevalence of C. scrofarum and C. suis infections among pigs could be a potential source of infection to humans.



中文翻译:

在泰国的猪中,由C. scrofarum和C. suis引起的隐孢子虫感染率很高。

隐孢子虫属。丙型肝炎是重要的肠道原生动物,可引起健康和免疫功能低下的患者,特别是艾滋病毒/艾滋病患者的腹泻。隐孢子虫属。可以通过食源,水源和人与人之间的途径传播。此外,几种隐孢子虫是人畜共患病的。本研究旨在确定泰国东部春武里府小农户(<50头/农场)和大型养殖场(50-500头/农场)饲养的猪中隐孢子虫感染的流行程度,采用巢式PCR扩增猪的小亚基。核糖体RNA(SSU-rRNA)基因。还进行了DNA测序以鉴定隐孢子虫的种类从11个养猪场收集了245个粪便样品。在小农户和大型养猪场中,隐孢子虫感染的总体患病率为20.8%(51/245)。≤6个月的猪中隐孢子虫感染的发生率显着高于> 6个月的猪(p  <.001)。在51个隐孢子虫阳性样本中,鉴定出了阴囊隐孢子虫(42/51,82.4%)和猪隐孢子虫(9/51,17.6%)。小于6个月大的猪与6个月以上大的猪相比,阴囊念珠菌感染的患病率显着更高(分别为20.7%和2.1%,p <.001)。的高患病率C. scrofarumC.猪的猪中的感染可能会传染给人类的潜在来源。

更新日期:2020-04-09
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