The N- and O-linked oligosaccharides from fission yeast Schizosaccharomyces pombe not only contain large amounts of d-mannose (Man) but also contain large amounts of d-galactose (Gal). Although the galactomannans of S. pombe are mainly composed of α1,2- or α1,3-linked Gals, some of the terminal α1,2-linked Gals are found to be linked to pyruvylated β1,3-linked galactose (PvGal). We have determined the structural characteristics of the N-glycans and O-glycans in three Schizosaccharomyces species (S. japonicus, S. octosporus, and S. cryophilus) using lectin blot, ¹H NMR spectroscopy, and size-fractionation high performance liquid chromatography (HPLC), and found that the galactosylation of oligosaccharides was a common feature in fission yeasts. In addition, each of the terminal Galα1,2-, Galβ1,3- and non-substituted Man residues exhibited distinct characteristics. A BLAST search of gene databases in Schizosaccharomyces identified genes homologous to pvg1 encoding pyruvyltransferase of S. pombe. These genes, when expressed in an S. pombe pvg1Δ strains, led to the pyruvylation of non-reducing terminal β-linked Gal, suggesting the biosynthetic pathway of PvGal-containing oligosaccharides is highly conserved in fission yeasts.

来自裂变酵母裂殖酵母的N-和O-连接的寡糖不仅含有大量的d-甘露糖（Man），而且含有大量的d-半乳糖（Gal）。尽管粟酒裂殖酵母的半乳甘露聚糖主要由与α1,2-或α1,3-连接的Gals组成，但发现一些末端与α1,2-连接的Gals与丙酮酸化的与β1,3-连接的半乳​​糖（PvGal）连接。我们已经确定了三种裂殖酵母属物种（S. japonicus，S。octosporus和S. cryophilus）中N-聚糖和O-聚糖的结构特征。），凝集素印迹，¹ H NMR光谱和大小分级高效液相色谱（HPLC），发现低聚糖的半乳糖基化是裂殖酵母的共同特征。另外，末端的Galα1,2-，Galβ1,3-末端和未取代的Man残基均表现出不同的特征。在裂殖酵母中对基因数据库进行了BLAST搜索，确定了与粟酒酵母丙酮酸转移酶pvg1同源的基因。这些基因在粟酒裂殖酵母pvg1Δ菌株中表达时，导致非还原性末端β-连接的Gal的丙酮酸化，表明含PvGal的寡糖的生物合成途径在裂殖酵母中高度保守。