2,3-Butanediol (2,3-BD) is a valuable platform chemical with extensive industrial applications. The demand for its safe and economic microbial synthesis resulted in increased interest in the isolation of non-pathogenic producers capable of converting cheap and renewable materials. This study reports the isolation of 62 new non-pathogenic Bacillus strains producing 2,3-BD. Three strains were found, including Bacillus velezensis 5RB, B. toyonensis 11RA and B. safensis 14A, which belonged to species not previously reported as 2,3-BD producers. Seventeen strains displayed cellulolytic activity, degrading carboxymethyl cellulose, HE-cellulose and β-glucan, whereas 5 strains were also able to hydrolyze arabinoxylan, arabinan, galactomannan, xyloglucan, xylan and galactan (included in hemicellulose), as well as starch and fructans. The strain capacity to ferment lignocellulosic sugars to 2,3-BD corresponded to the activities of CAZymes engaged in the hydrolysis of the relevant polysaccharides. Regardless of species, Bacillus strains converted glucose, cellobiose, and mannose to 2,3-BD with higher concentration, productivity and yield than arabinose, xylose, and galactose. B. velezensis 5RB was the only strain, which was capable to produce 2,3-BD from all lignocellulosic sugars which corresponded to the presence of the highest extracellular endo-α-(1→5)-l-arabinanase, endo-1,4-β-xylanase, 1,4-β-xylosidase, and endo-β-1,4-galactanase activity. The annotation of its genome showed the presence of 26 genes encoding glycoside-hydrolases allowing biomass degradation. In conclusion, the non-risk new isolated Bacillus strains are promising for 2,3-BD production from agro-industrial residues. When lignocellulose is used, the process would be more efficient if the substrate content is rich in cellulose and low in hemicelluloses.

2,3-丁二醇（2,3-BD）是一种有价值的平台化学品，具有广泛的工业应用。对其安全经济的微生物合成的需求引起了人们对隔离能够转化廉价和可再生材料的非致病性生产者的兴趣。这项研究报告了62种新的产生2,3-BD的非致病性芽孢杆菌菌株的分离。发现了三种菌株，包括velacensis velezensis 5RB，toyonensis B. toyonensis 11RA和safetysis B.14A，属于先前未报道为2,3-BD生产者的物种。17个菌株显示出纤维素分解活性，降解了羧甲基纤维素，HE-纤维素和β-葡聚糖，而5个菌株还能够水解阿拉伯木聚糖，阿拉伯聚糖，半乳甘露聚糖，木葡聚糖，木聚糖和半乳聚糖（包括在半纤维素中）以及淀粉和果聚糖。将木质纤维素糖发酵成2，3-BD的菌株能力对应于参与相关多糖水解的CAZymes的活性。不论物种如何，芽孢杆菌菌株均比阿拉伯糖，木糖和半乳糖将葡萄糖，纤维二糖和甘露糖以更高的浓度，生产率和产率转化为2,3-BD。velezensis5RB是唯一能够从所有木质纤维素糖中产生2,3-BD的菌株，这对应于最高的细胞外内切α-（1→5）-1-阿拉伯聚糖酶，内切1,4-β的存在-木聚糖酶，1,4-β-木糖苷酶和内切β-1,4-半乳聚糖酶活性。其基因组注释显示存在26个编码糖苷水解酶的基因，可降解生物质。总之，无风险的新分离芽孢杆菌菌株有望从农用工业残留物中生产2,3-BD。当使用木质纤维素时，如果底物含量富含纤维素而半纤维素含量低，则该方法将更加有效。