当前位置: X-MOL 学术J. Appl. Res. Med. Plants › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Simultaneous determination of baicalein, chrysin and wogonin in four Iranian Scutellaria species by high performance liquid chromatography
Journal of Applied Research on Medicinal and Aromatic Plants ( IF 3.9 ) Pub Date : 2019-11-09 , DOI: 10.1016/j.jarmap.2019.100232
Zahra Gharari , Khadijeh Bagheri , Hossein Danafar , Ali Sharafi

Scutellaria is a diverse and widespread genus of the Lamiaceae family of herbaceous plants known commonly as skullcaps. It has been implicated for medicinal purposes for the treatment of various ailments ranging from cancers, nervous disorders, hepatitis, anxiety and jaundice. A sensitive, simple and reliable high performance liquid chromatography (HPLC) method was developed for simultaneous quantification of three major active ingredients, chrysin, wogonin and baicalein in S. bornmuelleri, S. orientalis, S. araxensis and S. multicaulis. HPLC analysis was performed using a C8 analytical column. Detection was carried out at 262 nm using a mobile phase of methanol–acetonitrile–aqueous phosphoric acid at 1 ml min−1 flow rate. The test range of chrysin, wogonin and baicalein concentrations was from 0.5–400 μg mL−1. The experimental results revealed that the amount of baicalein in the roots of examined plants was more than shoots. Wogonin was more in the roots of S. orientalis and S. bornmuelleri and shoots of S. bornmuelleri and S. araxensis. The highest amount of chrysin was detected in the roots and shoots of S. orientalis and S. bornmuelleri. Totally the amount of flavonoids in S. orientalis shoot and root was more than other species. The high percentage of recovery (94.33–111.11 %), low limit of detection (LOD) and limit of quantitation (LOQ) and high R-squared value (R2 > 0.999) confirm the suitability of the method for simultaneous quantification of baicalein, chrysin and wogonin in the four Scutellaria species.



中文翻译:

高效液相色谱法同时测定伊朗四种S中的黄ical素,菊花素和伍格宁

黄cut唇形科的草本植物科的一种广泛而广泛的属,通常被称为黄cap。它已被暗示可用于治疗各种疾病,包括癌症,神经疾病,肝炎,焦虑症和黄疸。灵敏,简单和可靠的高效液相色谱(HPLC)方法对三个主要的活性成分,白杨素,汉黄芩素和黄芩素在同时定量开发S. bornmuelleriS.侧柏S. araxensisS. -多。使用C8分析柱进行HPLC分析。使用甲醇-乙腈-磷酸水溶液的流动相以1 ml min -1的流速在262 nm处进行检测。chrysin,wogonin和黄ical素的测试范围是0.5–400μgmL -1。实验结果表明,被检植物根中的黄ical素含量高于芽。汉黄芩素是更多的根S.侧柏S. bornmuelleri和芽S. bornmuelleriS. araxensis。在东方沙门氏菌天生沙门氏菌的根和芽中检测到最高的菊花素东方沙门氏菌芽和根中总的黄酮含量高于其他物种。高回收率(94.33–111.11%),低检测限(LOD)和定量限(LOQ)和高R平方值(R 2  > 0.999)证实了该方法适用于同时进行黄ical素定量分析,四种cut中的金霉素和沃贡宁。

更新日期:2019-11-09
down
wechat
bug