Wilt disease of isabgol (Plantago ovata Forsk.), caused by Fusarium oxysporum species complex is one of the economically important disease in India. The pathogen survive in soil on plant debris and invaded crop plants at any stage from germination to maturity and drastically imposed the production. The disease incidence influenced by several agronomical and environmental factors and varied from 10 to 60 % prevalence. The present study was carried out to confirm the pathogenicity of associated Fusarium species of wilt disease, characterization using the morphological and molecular tools and devise the of management strategies. The Fusarium oxysporum species complex induced typical wilt symptoms on mature isabgol plants whereas, damping off like symptoms on the seedling stage. The fungal colony appeared white to purplish with cottony mycelium growth on PDA. Macroconidia were oval to slightly curved shape and septate in 2–4 cells with tapering pointed ends. The phylogenetic tree constructed based on partial ITS, translation elongation factor (EF-1α) and RNA polymerase II (RPB2) and Fusarium MLTS database confirmed the association of Fusarium oxysporum species complex with the wilt disease of P. ovata. Seed dressing using carbendazim (50 W P) along with the soil application of Trichoderma viride enriched neem cake mixture before sowing significantly reduce the disease incidence. To the best of our knowledge, this is the first comprehensive report on molecular characterization of Fusarium oxysporum associated with wilt disease of P. ovata.

由尖孢镰刀菌种复合物引起的伊萨布固醇（Plantago ovata Forsk。）枯萎病是印度经济上重要的疾病之一。从发芽到成熟的任何阶段，病原体都可以在土壤中的植物残骸和入侵的作物上生存，并极大地影响了生产。该疾病的发病率受多种农学和环境因素的影响，患病率从10％到60％不等。本研究旨在确定相关的枯萎病镰刀菌的致病性，使用形态学和分子工具进行表征，并制定管理策略。在尖孢镰刀菌种复合物在成熟的isabgol植物上引起典型的枯萎症状，而在幼苗期则逐渐消失。真菌菌落看起来为白色至略带紫色，并在PDA上出现棉质菌丝体。大分生孢子体呈椭圆形至略微弯曲的形状，并在2-4个细胞中分离，尖端逐渐变细。基于部分ITS，翻译延伸因子（EF-1α）和RNA聚合酶II（RPB2）以及镰刀菌MLTS数据库构建的系统树证实了尖孢镰刀菌种复合体与卵形青霉病有关。使用多菌灵（50 WP）拌种以及在土壤中施用木霉播种前富含印度ne饼混合物可显着降低疾病发病率。据我们所知，这是首次报道与枯萎病相关的尖孢镰刀菌分子特征的综合报告。