Melanoma is one of the major types of skin cancer. The metastatic melanoma is among the most lethal forms of malignant skin tumors. We hereby aimed to characterize a novel microRNA (miR) in the metastatic melanoma model. First, we evaluated the expression of miR-107 in melanoma cells and tumor tissues. The comparison between primary and metastatic cancer tissues was also accessed. Next, we examined the impact of miR-107 on melanoma cell proliferation, cell cycle, colony formation, apoptotic activity, migration and matrix invasion. A downstream target of miR-107 was also predicted and validated functionally in melanoma cells. Our findings showed miR-107 was significantly downregulated in melanoma. Its expression was lowest in metastatic form. Over-expression of miR-107 reduced melanoma cell proliferation, migration and invasion. POU3F2 was identified as the downstream target of miR-107. Over-expression of POU3F2 antagonized miR-107-mediated inhibitory effect on melanoma cells. Our study has reported miR-107 as a novel tumor suppressive factor in the metastatic melanoma model. It has provided new avenue to manage melanoma and improve the survival rate in the advanced stage.

中文翻译：

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MicroRNA-107是靶向黑色素瘤中POU3F2的新型肿瘤抑制剂

黑色素瘤是皮肤癌的主要类型之一。转移性黑色素瘤是恶性皮肤肿瘤最致命的形式之一。我们在此旨在表征转移性黑色素瘤模型中的新型microRNA（miR）。首先，我们评估了miR-107在黑素瘤细胞和肿瘤组织中的表达。还进行了原发性和转移性癌组织之间的比较。接下来，我们检查了miR-107对黑素瘤细胞增殖，细胞周期，集落形成，凋亡活性，迁移和基质侵袭的影响。还预测了miR-107的下游靶标，并在黑色素瘤细胞中进行了功能验证。我们的研究结果表明，miR-107在黑色素瘤中显着下调。其表达以转移形式最低。miR-107的过表达减少了黑色素瘤细胞的增殖，迁移和侵袭。POU3F2被确定为miR-107的下游目标。POU3F2的过表达拮抗miR-107介导的对黑素瘤细胞的抑制作用。我们的研究报告了miR-107作为转移性黑色素瘤模型中的一种新型肿瘤抑制因子。它为晚期黑色素瘤的治疗和提高生存率提供了新途径。