A homogeneous monomeric laccase (ASL) from Agaricus sinodeliciosus, with a molecular mass of 65 kDa, was isolated using ion‐exchange chromatography (CM‐cellulose and Q‐Sepharose) and gel‐filtration chromatography (Superdex 75). This laccase exhibited maximum activity at 50 °C and pH 5.0. Hg²⁺ and Cd²⁺ significantly inhibited its activity. The laccase displayed a K_m value of 0.9 mM toward 2,2′‐azinobis‐(3‐ethylbenzthiazoline‐6‐sulfonate) (ABTS). In addition to ABTS, ASL exhibited higher affinity toward o‐toluidine and benzidine than other substrates. ASL is able to decolorize malachite green and Eriochrome black T.

中文翻译：

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蘑菇姬松茸漆酶的纯化及性质

使用离子交换色谱法（CM-纤维素和Q-Sepharose）和凝胶过滤色谱法（Superdex 75）分离出来自姬松茸的均质单体漆酶（ASL），其分子量为65 kDa。该漆酶在50℃和pH 5.0下表现出最大的活性。Hg ²⁺和Cd ²⁺显着抑制其活性。漆酶对2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS）的K _m值为0.9 mM。除ABTS外，ASL对邻甲苯胺和联苯胺的亲和力也比其他底物高。ASL能够使孔雀石绿和铬黑色T脱色。