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Fabrication of penicillin G portable microbiological test plate for on-farm antibiotic residues in milk
Journal of Food Measurement and Characterization ( IF 3.4 ) Pub Date : 2020-04-13 , DOI: 10.1007/s11694-020-00456-7
Moeketsi Ntakatsane , Ping Chen , Jingsheng Liu , Linlin Xu , Na Dong , Poloko Mosebi , George Adoko , Puleng Matebesi

Antibiotic residues in animal-derived food products such as milk pose a potential health risk to consumers. A portable antimicrobial screening test plate was fabricated particularly for on-farm testing of milk. A cold-water soluble compound gelling agent composed of guar gum (GG) and konjac glucomannan (KG) was prepared and its properties were optimized for enhancing performance of the test plate. A ratio of 4:6 (GG:KG) showed improved gel strength, cohesiveness, transmittance, and water diffusion of the compound gel. Microbial colony number and size were also increased. The test plate displayed a minimum inhibition concentration of 4 µg/L penicillin G and clearer zone of inhibition. Detection time was also significantly reduced to approximately 9–15 h compared to the conventional agar plate method. The plastic film test plate with optimized compound gel demonstrated tremendous potential for detection of antibiotics in milk.

中文翻译:

青霉素G便携式牛奶农场抗生素残留微生物试验板的研制。

动物性食品(例如牛奶)中的抗生素残留会对消费者构成潜在的健康风险。制造了一种便携式抗菌筛查测试板,专门用于农场牛奶的现场测试。制备了由瓜尔胶(GG)和魔芋葡甘露聚糖(KG)组成的冷水溶性复合胶凝剂,并对其性能进行了优化,以增强测试板的性能。4:6(GG:KG)的比例显示出复合凝胶的改善的凝胶强度,内聚性,透射率和水扩散性。微生物菌落的数量和大小也增加了。该测试板显示的最小抑菌浓度为4 µg / L青霉素G,抑菌区更清晰。与传统的琼脂平板法相比,检测时间也显着减少至大约9-15小时。
更新日期:2020-04-13
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