This study sought to investigate the effects caused by the application of supercritical CO₂ treatment in the commercial cellulase Celluclast 1.5L. The influence of this treatment on the enzymatic hydrolysis of sugarcane bagasse (2% w v⁻¹) and on the kinetic parameters was assessed in a central composite design (CCD). The results show that the application of supercritical treatment for 180 min at 40 °C and 300 bar causes an increase in 14.31% in total cellulase enzymatic activity (FPase). Furthermore, this treatment conditions also enhance in 73.63% (concentration of 1.176 g L⁻¹) the concentration of fermentable sugars released during the enzymatic hydrolysis of sugarcane bagasse, with theoretical yield of cellulose hydrolysis of 17.56%. In addition, K_M was reduced 3 times for the enzyme compared to the untreated enzyme. Thus, treatment with supercritical CO₂ proved to be a green and attractive alternative for fermentable sugars release, since it resulted in enhancements of enzymatic activities of the cellulolytic complex and an increase in enzymatic hydrolysis degree of the sugarcane bagasse.

中文翻译：

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超临界CO 2处理的应用增强了甘蔗渣的酶促水解

这项研究旨在研究在商业纤维素酶Celluclast 1.5L中应用超临界CO ₂处理所引起的影响。在中央复合设计（CCD）中评估了该处理对甘蔗渣（2％w v ^-1）的酶促水解和动力学参数的影响。结果表明，在40°C和300 bar下进行180分钟的超临界处理会使总纤维素酶的酶促活性（FPase）增加14.31％。此外，该处理条件还提高了在甘蔗渣的酶促水解过程中释放的可发酵糖的浓度，为73.63％（浓度为1.176 g L ^-1），纤维素水解的理论收率为17.56％。另外，K与未处理的酶相比，该酶的_M降低了3倍。因此，用超临界CO _2处理被证明是可发酵糖释放的绿色和有吸引力的替代方法，因为它导致纤维素分解复合物的酶活性增强和甘蔗渣的酶水解度增加。