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Diagnostic amide products of amino lipids detected in the microaerophilic bacteria Lutibacter during routine fatty acid analysis using gas chromatography
Organic Geochemistry ( IF 3 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.orggeochem.2020.104027
Nicole J. Bale , Michel Koenen , Subhash Yadav , Ellen C. Hopmans , Laura Villanueva , Jaap S. Sinninghe Damsté , Stefan Schouten

Abstract Analysis of fatty acids in the form of fatty acid methyl esters (FAMEs) using gas chromatography (GC) is routine within microbiology but still some compounds remain unidentified. During characterization of the FAMEs of two strains of the microaerophilic bacterium Lutibacter sp., recently isolated from the Black Sea, a series of compounds, eluting after the regular FAMEs, were detected. We identified these compounds using GC–mass spectrometry (GC–MS) and an authentic standard, to be amino acids glycine-linked via an amide bond to β-hydroxy fatty acids (i.e. glycine β-hydroxy fatty acid amides). Analysis of the intact polar lipids of the Lutibacter species by ultra-high performance liquid chromatography–high resolution mass spectrometry (UHPLC–HRMS) showed that the glycine β-hydroxy fatty acid amides are derived from glycine lipids (also known as cytolipins), which are amino acid lipids. Amino acid lipids represent an under-studied, but potentially significant, group of microbial membrane lipids and our results provide a rapid way to detect the presence of glycine lipids during routine fatty acid analysis by GC. Furthermore, glycine β-hydroxy fatty acid amides represent easily detectable biomarker lipids for glycine lipid-producing microorganisms in natural environments.

中文翻译:

在使用气相色谱法进行常规脂肪酸分析期间在微需氧细菌 Lutibacter 中检测到的氨基脂质的诊断性酰胺产物

摘要 使用气相色谱 (GC) 分析脂肪酸甲酯 (FAME) 形式的脂肪酸是微生物学中的常规方法,但仍有一些化合物尚未鉴定。在对最近从黑海分离的微需氧细菌 Lutibacter sp. 的两种菌株的 FAME 进行表征期间,检测到一系列化合物,在常规 FAME 之后洗脱。我们使用 GC-质谱法 (GC-MS) 和可靠的标准鉴定了这些化合物,这些化合物是通过酰胺键与 β-羟基脂肪酸(即甘氨酸 β-羟基脂肪酸酰胺)连接的氨基酸甘氨酸。通过超高效液相色谱-高分辨质谱 (UHPLC-HRMS) 分析 Lutibacter 物种的完整极性脂质表明甘氨酸 β-羟基脂肪酸酰胺来源于甘氨酸脂质(也称为细胞脂),是氨基酸脂质。氨基酸脂质代表了一组研究不足但具有潜在意义的微生物膜脂质,我们的结果提供了一种在常规脂肪酸分析过程中检测甘氨酸脂质存在的快速方法。此外,甘氨酸β-羟基脂肪酸酰胺代表了自然环境中产生甘氨酸脂质的微生物的易于检测的生物标志物脂质。微生物膜脂组和我们的结果提供了一种在 GC 常规脂肪酸分析过程中检测甘氨酸脂质存在的快速方法。此外,甘氨酸β-羟基脂肪酸酰胺代表了自然环境中产生甘氨酸脂质的微生物的易于检测的生物标志物脂质。微生物膜脂组和我们的结果提供了一种在 GC 常规脂肪酸分析过程中检测甘氨酸脂质存在的快速方法。此外,甘氨酸β-羟基脂肪酸酰胺代表了自然环境中产生甘氨酸脂质的微生物的易于检测的生物标志物脂质。
更新日期:2020-06-01
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