The microalga Dunaliella salina was studied at the main stages of transition from laboratory to pilot-scale cultivation: strain selection, nutrient medium selection, estimation of influence of physicochemical factors on accumulation of carotenoids and evaluation of the selected strain growing technology at pilot conditions. Dunaliella salina strain IBSS-2 was recognized as promising for commercial cultivation due to the combination of high-production characteristics, environmental stress resistance, and relative easiness of transition to the carotenogenesis stage. The influence of stress factors on the D. salina culture productivity in two nutrient media was estimated. It was shown that light effect combined with nutrients deficiency is the key factor for β-carotene accumulation. The influence of increased irradiance caused the increase of carotenoid content in D. salina cells up to 8%, and increasing irradiance and salinity resulted in carotenoid productivity going up 1.5 times. Testing of D. salina pilot cultivation system demonstrated that productivity at the first cultivation stage was about 6 g m⁻² day⁻¹ in both batch and semicontinuous mode. During pilot D. salina cultivation in Crimea, the culture transition to the carotenogenesis stage was achieved both in summer and autumn. The concentration of carotenoids in the ponds was 200 and 600 mg m⁻² with a carotenoid/chlorophyll a (Car/Chl a) ratio of 7 and 4.5 in summer and autumn, respectively. The possibility to use natural population of D. salina cells from salterns as inoculum and brine as a nutrient medium base was demonstrated. The study results suggest that the proposed approach can be recommended for D. salina commercial cultivation.

微藻杜氏盐藻在从实验室到中试规模种植的主要阶段进行了研究：菌株选择，营养培养基选择，理化因素对类胡萝卜素积累的影响的估计以及在试验条件下评估所选菌株的生长技术。杜氏盐藻盐藻菌株IBSS-2由于具有高产量特性，耐环境胁迫性和相对容易过渡到胡萝卜素形成阶段的优点，因此被认为可用于商业化种植。压力因素对盐沼石。的影响估计了两种营养培养基的培养生产力。结果表明，光效应与营养物质缺乏是β-胡萝卜素积累的关键因素。辐照度增加的影响导致盐藻中类胡萝卜素含量增加了8％，辐照度和盐度的增加使类胡萝卜素的生产率提高了1.5倍。D. salina中试栽培系统的测试表明，在分批和半连续模式下，第一栽培阶段的生产力约为6 g m ^-2  day ^-1。在飞行员D.萨利纳期间在克里米亚种植，在夏季和秋季都实现了向胡萝卜素形成阶段的文化过渡。池塘中类胡萝卜素的浓度分别为200和600 mg m ^-2，夏季和秋季的类胡萝卜素/叶绿素a（Car / Chl a）之比分别为7和4.5。证明了有可能使用来自盐厂的天然盐生梭状芽胞杆菌细胞作为接种物，并使用盐水作为营养培养基。研究结果表明，所建议的方法可以推荐用于盐藻的商业化种植。