Urban waste is a complex mixture of different substances, including microplastics and pharmaceuticals and personal care products. Microplastics have a high affinity for hydrophobic substances. One of these substances is triclosan, a bactericide used in a variety of hygiene products. Therefore, microplastics (MPs) may serve as a vector between triclosan and aquatic organisms. The current study sought to evaluate the effects of the interaction between microplastics and triclosan based on a mechanistic approach in which the oyster Crassostrea brasiliana was used as a model. The organisms were exposed to three conditions: the control, microplastic (MP), and microplastic contaminated with triclosan (MPT). The organisms were exposed for 3 or 7 days. After the exposure time, hemolymph was sampled for performing the neutral red retention time assay and, subsequently, the gills, digestive glands, and adductor muscles were dissected for measuring biomarkers responses (EROD, DBF, GST, GPx, GSH, lipid peroxidation, DNA strand breaks, and AChE). Our results demonstrate combined effects of MPs associated with triclosan on oyster physiology and biochemistry, as well as on lysosomal membrane stability. These results contribute to understanding the effects of contaminants of emerging concern and microplastics on aquatic organisms.

城市垃圾是各种物质的复杂混合物，包括微塑料，药品和个人护理产品。微塑料对疏水性物质具有很高的亲和力。这些物质之一是三氯生，一种用于多种卫生产品的杀菌剂。因此，微塑料（MPs）可以作为三氯生和水生生物之间的媒介。当前的研究试图基于一种机制方法来评估微型塑料与三氯生之间的相互作用，其中牡蛎Crassostrea brasiliana被用作模型。生物体暴露于三种条件下：对照，微塑性（MP）和被三氯生污染的微塑性（MPT）。将该生物暴露3或7天。在暴露时间之后，取样血淋巴以进行中性红色保留时间测定，然后解剖dis，消化腺和内收肌，以测量生物标志物的响应（EROD，DBF，GST，GPx，GSH，脂质过氧化，DNA链断裂和AChE）。我们的结果证明了与三氯生相关的MP对牡蛎生理和生化以及溶酶体膜稳定性的综合影响。这些结果有助于理解新兴关注的污染物和微塑料对水生生物的影响。