Interferon regulatory factor 7 (IRF7) serves as a critical mediator in the regulation of type Ι interferon (IFN) response to invading pathogens. Here, an ortholog of IRF7 was characterized in yellow catfish (Pelteobagrus fulvidraco). The full-length cDNA of PfIRF7 consisted of 1516 bp encoding a polypeptide of 425 amino acids. PfIRF7 protein comprised a typical IRF structural architecture, including a DNA binding domain (DBD), an IRF association domain (IAD) and a serine-rich domain (SRD). PfIRF7 was expressed predominantly in the immune-related tissues and transcriptionally upregulated by PolyI:C, LPS, and Edwardsiella ictaluri. Ectopic expression of PfIRF7 led to activation of fish type I IFN promoters and induction of IFN and Vig1, thereby conferring a strong antiviral effect against spring viremia of carp virus (SVCV). Overall, the present data suggest that PfIRF7 may play an essential role in type I IFN response of yellow catfish.

中文翻译：

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黄鲶 (Pelteobagrus fulvidraco) IRF7 的分子特征表明参与了先天免疫反应。

干扰素调节因子 7 (IRF7) 在调节 1 型干扰素 (IFN) 对入侵病原体的反应中起着关键介质的作用。在这里，IRF7 的直系同源物在黄鲶 (Pelteobagrus fulvidraco) 中得到表征。PfIRF7 的全长 cDNA 由 1516 bp 组成，编码 425 个氨基酸的多肽。PfIRF7 蛋白包含典型的 IRF 结构结构，包括 DNA 结合域 (DBD)、IRF 关联域 (IAD) 和富含丝氨酸的域 (SRD)。PfIRF7 主要在免疫相关组织中表达，并被 PolyI:C、LPS 和 Edwardsiella ictaluri 转录上调。PfIRF7 的异位表达导致鱼 I 型 IFN 启动子的激活和 IFN 和 Vig1 的诱导，从而对鲤鱼病毒（SVCV）的春季病毒血症具有很强的抗病毒作用。全面的，