Silybum marianum (L.) Gaertn. (Asteraceae) was hydroponically cultured using a nutrient film technique system. Silibinin, isosilibinin and silychristin were detected in the fruits of the cultured plants. The effect of salicylic acid on the improvement of flavonolignans production by the fruits of the hydroponically cultured S. marianum was investigated. Salicylic acid was added to the nutrient solution at different concentrations (100, 200 and 400 µM) and the mature fruits of the plant were collected five days after elicitor addition. The fruits were then analyzed for their total flavonolignans contents and individual components using quantitative proton nuclear magnetic resonance spectroscopy (qHNMR) and high-performance liquid chromatography (HPLC). The results showed that elicitation with salicylic acid at 200 µM for five days increased production of total flavonolignans (1.7-fold by qHNMR and 1.6-fold by HPLC) higher than the control cultures and (1.4-fold by qHNMR and 1.1-fold by HPLC) higher than the cultivated plants. Silychristin was the major flavonolignan produced by the cultured plant. Elicitation by 200 µM salicylic acid increased silychristin production (1.6-fold by qHNMR and HPLC) higher than the control cultures and (1.3-fold by qHNMR and 1.0-fold by HPLC) higher than the cultivated plants. The present study provides a chance to improve secondary metabolite yield, serves as a useful tool for studying the biosynthesis of these medicinally valuable compounds and its regulation in plant and spots more light on hydroponic system as an important agricultural technique.

水飞蓟（L.）Gaertn。（Asteraceae）使用营养膜技术系统进行水培。在培养植物的果实中检测到水飞蓟宾，异水飞蓟宾和水飞蓟素。水杨酸对水培S果实改善黄酮生成的影响。马里亚努姆被调查了。将水杨酸以不同浓度（100、200和400 µM）添加到营养液中，并在添加引发剂5天后收集植物的成熟果实。然后使用定量质子核磁共振波谱（qHNMR）和高效液相色谱（HPLC）分析水果的总黄酮含量和各个成分。结果表明，以200 µM的水杨酸激发5天，总黄酮木聚糖的产量（qHNMR为1.7倍，HPLC为1.6倍）高于对照培养物（qHNMR为1.4倍，HPLC为1.1倍） ）高于栽培植物。水飞蓟素是培养植物产生的主要黄素。用200 µM水杨酸进行激发可提高水飞蓟宾产量（1。qHNMR和HPLC的结果是对照培养物的6倍，qHNMR的结果是1.3倍，HPLC的结果是1.0倍。本研究提供了提高次生代谢产物产量的机会，可作为研究这些具有药用价值的化合物的生物合成及其在植物中的调控的有用工具，并为水培系统作为一种重要的农业技术增色不少。