The differentiation of cultured Bone marrow stromal cells (BMSC) on silk scaffold into mature oligodendrocyte was done in the presence of cerebrospinal fluid (CSF). BMSC were isolated from Sprague-Dawley rats and were seeded on silk scaffold. The seeded cells were cultured in DMEM/F12 medium supplemented with CFS, basic fibroblast growth factor (bFGF), Retinoic acid (RA) and Epidermal growth factor (EGF). The glial differentiation was investigated using Real time-PCR and immunofluorescence techniques for specific glial markers: Oligo 2, NG2, PLP and MBP. Our dates showed that the differentiated cells expressed specific glial markers: Oligo 2, NG2, PLP and MBP. The specific mature oligodendrocyte genes were up regulated in cultured cells on silk scaffold in the presence of CSF. It is concluded that CSF leads to improve glial differentiation of seeded BMSC on silk scaffold using preparation of appropriate niche. This culture condition may be served as an efficient differentiation induction protocol for glial phenotype, with the perspective of therapeutic application in neuroregenerative medicine.

中文翻译：

---

使用脑脊液将播种在丝支架上的骨髓基质干细胞分化为成熟的少突胶质细胞

在脑脊液 (CSF) 存在下，将丝支架上培养的骨髓基质细胞 (BMSC) 分化为成熟的少突胶质细胞。BMSC 是从 Sprague-Dawley 大鼠中分离出来的，并接种在丝支架上。接种的细胞在补充有 CFS、碱性成纤维细胞生长因子 (bFGF)、视黄酸 (RA) 和表皮生长因子 (EGF) 的 DMEM/F12 培养基中培养。使用实时 PCR 和免疫荧光技术对特定神经胶质标记物：Oligo 2、NG2、PLP 和 MBP 研究神经胶质分化。我们的日期显示分化的细胞表达特定的神经胶质标志物：Oligo 2、NG2、PLP 和 MBP。在存在脑脊液的情况下，丝支架上培养的细胞中特定的成熟少突胶质细胞基因上调。得出的结论是，使用适当的生态位制备，CSF 可改善丝支架上接种的 BMSC 的神经胶质分化。从神经再生医学治疗应用的角度来看，这种培养条件可作为神经胶质表型的有效分化诱导方案。