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Circular RNA has_circ_0000527 participates in proliferation, invasion and migration of retinoblastoma cells via miR-646/BCL-2 axis.
CELL BIOCHEMISTRY AND FUNCTION ( IF 3.6 ) Pub Date : 2020-04-07 , DOI: 10.1002/cbf.3535
Nan-Nan Chen 1 , Dai-Ling Chao 2 , Xiu-Gui Li 2
Affiliation  

Retinoblastoma (RB) is one of the most common primary intraocular malignancies in children. Emerging researches have shown that circular RNAs (circRNAs) play critical roles in a variety of cancers. As a novel circRNA, the function of circ_0000527 in RB remains unknown. In this work, expression level of circ_0000527 and miR‐646 in RB tissues and cell lines were detected by quantitative real‐time polymerase chain reaction (qRT‐PCR). RB cell lines (SO‐Rb50 and WERI‐Rb‐1) were used as cell models in functional experiments. CCK‐8 assay, TUNEL assay and transwell assay were employed to detect the biological influence of circ_0000527 and miR‐646 on cancer cells in vitro. qRT‐PCR, luciferase reporter assay, RIP assay and western blot were used for exploring the interactions among circ_0000527, miR‐646 and BCL‐2. It was demonstrated that expression level of circ_0000527 in RB samples was significantly up‐regulated compared to normal tissues, while miR‐646 was markedly down‐regulated. Overexpression of circ_0000527 promoted the viability, migration and invasion of RB cells, while miR‐646 transfection had the opposite effects. Circ_0000527 sponged miR‐646 to regulate the expression of BCL‐2. In conclusion, circ_0000527 could promote the development of RB by indirectly modulating BCL‐2 via absorbing miR‐646.

中文翻译:

环状RNA has_circ_0000527通过miR-646 / BCL-2轴参与成视网膜细胞瘤细胞的增殖,侵袭和迁移。

视网膜母细胞瘤(RB)是儿童中最常见的原发性眼内恶性肿瘤之一。新兴研究表明,环状RNA(circRNA)在多种癌症中起着至关重要的作用。作为一种新型的circRNA,circ_0000527在RB中的功能仍然未知。在这项工作中,通过定量实时聚合酶链反应(qRT-PCR)检测了RB组织和细胞系中circ_0000527和miR‐646的表达水平。RB细胞系(SO-Rb50和WERI-Rb-1)在功能实验中用作细胞模型。使用CCK-8,TUNEL和Transwell分析检测circ_0000527和miR-646对体外癌细胞的生物学影响。使用qRT-PCR,荧光素酶报告基因分析,RIP分析和Western blot来研究circ_0000527,miR-646和BCL-2之间的相互作用。结果表明,与正常组织相比,RB样品中circ_0000527的表达水平显着上调,而miR-646显着下调。circ_0000527的过表达促进了RB细胞的活力,迁移和侵袭,而miR-646转染则具有相反的作用。Circ_0000527用海绵对miR-646进行调节,以调节BCL-2的表达。总之,circ_0000527可通过吸收miR-646间接调节BCL-2来促进RB的发展。
更新日期:2020-04-07
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