Growth hormone (GH) excess in bovine (b)GH transgenic mice has been shown to alter white adipose tissue (WAT) immune cell populations. The present study aimed to evaluate the effects of GH resistance on WAT immune cell populations using GH receptor knockout (GHR^−/−) mice. Eight‐ and 24‐month‐old, male GHR^−/− and wild‐type mice were used. Body composition and tissue weights were determined, and systemic inflammation was assessed by measuring serum cytokine levels. The stromal vascular fraction (SVF) was isolated from three distinct WAT depots, and immune cell populations were quantified using flow cytometry. GHR^−/− mice at both ages had decreased body weight but were obese. Although no significant changes were observed in serum levels of the measured cytokines, SVF cell alterations were seen and differed from depot to depot. Total SVF cells were decreased in epidydimal (Epi) depots, whereas SVF cells per gram adipose tissue weight were increased in mesenteric (Mes) depots of GHR^−/− mice relative to controls. T cells and T helper cells were increased in Mes at 8 months old, whereas cytotoxic T cells were decreased in subcutaneous (SubQ) at 24 months old. Other cells were unchanged at both ages measured. The present study demonstrates that removal of GH action results in modest and depot‐specific changes to several immune cell populations in WAT of intra‐abdominal depots (Epi and Mes), which are somewhat surprising results because the SubQ has the largest change in size, whereas the Mes has no size change. Taken together with previous results from bovine GH transgenic mice, these data suggest that GH induces changes in the immune cell population of WAT in a depot‐specific manner. Notably, GHR^−/− mice appear to be protected from age‐related WAT inflammation and immune cell infiltration despite obesity.

中文翻译：

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GHR-/- 小鼠免受与肥胖相关的白色脂肪组织炎症的影响。

牛 (b) GH 转基因小鼠的生长激素 (GH) 过量已被证明会改变白色脂肪组织 (WAT) 免疫细胞群。本研究旨在使用 GH 受体敲除 (GHR ^-/- ) 小鼠评估 GH 抗性对 WAT 免疫细胞群的影响。使用了 8 个月和 24 个月大的雄性 GHR ^-/-和野生型小鼠。确定身体成分和组织重量，并通过测量血清细胞因子水平评估全身炎症。从三个不同的 WAT 库中分离出基质血管部分 (SVF)，并使用流式细胞术量化免疫细胞群。GHR ^-/-两个年龄的小鼠体重都下降了，但肥胖了。尽管在测量的细胞因子的血清水平中没有观察到显着变化，但是观察到了 SVF 细胞改变，并且每个仓库都不同。附睾 (Epi) 贮库中的总 SVF 细胞减少，而 GHR ^-/-肠系膜 (Mes) 贮库中每克脂肪组织重量的 SVF 细胞增加小鼠相对于对照。8 个月大时 Mes 中的 T 细胞和 T 辅助细胞增加，而 24 个月大时皮下 (SubQ) 中的细胞毒性 T 细胞减少。其他细胞在测量的两个年龄都没有变化。本研究表明，去除 GH 作用会导致腹内仓库（Epi 和 Mes）的 WAT 中几种免疫细胞群发生适度和仓库特异性的变化，这有点令人惊讶，因为 SubQ 的大小变化最大，而 Mes 没有大小变化。结合之前来自牛 GH 转基因小鼠的结果，这些数据表明 GH 以仓库特异性方式诱导 WAT 免疫细胞群的变化。值得注意的是，GHR ^-/- 尽管肥胖，小鼠似乎可以免受与年龄相关的 WAT 炎症和免疫细胞浸润的影响。