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Analysis of deformable distortion in the architecture of leaf xylary vessel elements of Carthamus oxycantha caused by heavy metals stress using image registration.
Microscopy Research and Technique ( IF 2.5 ) Pub Date : 2020-03-31 , DOI: 10.1002/jemt.23476
Tahir Iqbal 1 , Said K Shah 2 , Faizan Ullah 1 , Sultan Mehmood 1 , Muhammad A Zeb 1
Affiliation  

Anatomical study of leaf xylary vessel elements of Carthamus oxycantha under various intensities of lead (Pb) and nickel (Ni) stress (200, 400, 600, and 800 mg Pb(NO3)2, NiCl2·6H2O/kg of the soil) was conducted. The deformations caused due to metal stress were detected using point‐based image registration technique. Initially, a set of corresponding feature points called landmarks was selected for warping of two‐dimensional microscopic images of deformed/source vessel (stressed) to its normal/target (unstressed) counterpart. The results show that the target registration error is less than 3 mm using real plant image datasets. The stress caused alterations mainly in diameter, size, and shape of the cells. Average cell diameter and average wall diameter of vessels were measured with “Image J.” The range of decrease in average cell diameter from 18.566 to 13.1 μm and the range of increase in average wall diameter was from 5.166 to 10.1 μm, with increase in stress factor through 200, 400, 600, and 800 mg Pb(NO3)2, NiCl2·6H2O/kg of the soil. We noted large deformation in the form of shrinkage in cell size and diminution in its diameter. The diminution in diameter and the shrinkage in cell size of vessel cells may be due to the deposition of wall materials. It can be a possible strategy to limit the water flow to overcome the rapid mobility and transportation of the excess amount of metals to safeguard the cellular components from unpleasant consequences of metallic stress.

中文翻译:

利用图像配准分析重金属胁迫引起的红花叶片木叶脉管元件结构中的变形畸变。

的叶xylary容器元件的解剖学研究红花oxycantha下的铅各种强度(Pb)和镍(Ni)应力(200,400,600,和800毫克的Pb(NO 32,的NiCl 2 ·6H 2O / kg土壤。使用基于点的图像配准技术检测到由金属应力引起的变形。最初,选择了一组称为地标的相应特征点,以将变形/源血管(受力)的二维显微图像扭曲到其法线/目标(无应力)对应物。结果表明,使用真实植物图像数据集,目标配准误差小于3 mm。应力引起的改变主要是细胞的直径,大小和形状。用“ Image J”测量血管的平均细胞直径和平均壁直径。Pb(NO,200,400,600和800 mg的应力因数增加,平均泡孔直径的减小范围从18.566到13.1μm,平均壁直径的增大范围从5.166到10.1μm32,的NiCl 2 ·6H 2 O / kg的土壤中的。我们注意到以细胞尺寸缩小和直径减小的形式出现了较大的变形。血管细胞的直径减小和细胞尺寸缩小可能是由于壁材料的沉积所致。限制水流以克服过量金属的快速迁移和运输以保护细胞组分免受金属应力的不利影响可能是一种可能的策略。
更新日期:2020-03-31
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