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Compositional characterization of Pyrus ussuriensis Maxim and their antioxidant activities and induction of apoptosis in Bel-7402 cell.
Journal of Food Biochemistry ( IF 4 ) Pub Date : 2020-04-08 , DOI: 10.1111/jfbc.13222
Fei Peng 1, 2 , Gang Li 1 , Ying Xie 1, 2 , Hong-Yang Yin 1 , Xiao-Jing Li 1, 2 , Yue-Dong Yang 1, 2
Affiliation  

In this work, a comparison study on active sites (the total phenolic, total flavonoids, and total triterpenes contents) and antioxidant activities (DPPH, ABTS, and FRAP assays) of different fractions from Pyrus ussuriensis Maxim was evaluated. Moreover, the inhibition capability on human hepatocarcinoma cells Bel‐7402 cells and the mechanism was also discussed. Results showed that the ethyl acetate fraction significantly scavenged DPPH and ABTS+ radicals, exhibited ferric ion reducing antioxidant, and inhibited Bel‐7402 cells proliferation. In addition, oleanolic acid was the dominant compound act on the Bel‐7402 cells in the extract and it induced apoptosis by the caspase pathway and induced cell cycle arrest at the G0/G1 phase by inhibiting the cyclin D1/CDK4 pathway. The extracts of P. ussuriensis Maxim were confirmed to have anti‐oxidative and antiproliferative effects against Bel‐7402 cell in vitro.

中文翻译:

梨Pyrus ussuriensis Maxim的组成特征及其抗氧化活性和诱导Bel-7402细胞凋亡。

在这项工作中,评估了对Pyrus ussuriensis Maxim的不同馏分的活性部位(总酚,总黄酮和总三萜含量)和抗氧化活性(DPPH,ABTS和FRAP测定)的比较研究。此外,还讨论了对人肝癌细胞Bel-7402细胞的抑制能力及其机理。结果表明,乙酸乙酯级分可明显清除DPPH和ABTS +自由基,表现出铁离子还原抗氧化剂,并抑制Bel‐7402细胞增殖。此外,齐墩果酸是作用于提取物中Bel-7402细胞的主要化合物,它通过caspase途径诱导凋亡,并通过抑制细胞周期蛋白D1 / CDK4途径诱导细胞周期停滞在G0 / G1期。经证实,ussuriensis Maxim的提取物对Bel-7402细胞具有抗氧化和抗增殖作用。
更新日期:2020-04-08
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