The aim of this study was to investigate the neuroprotective role of ellagic acid (EA) on CCl₄‐induced brain injury in rats. In this study, the rats were divided into four groups. Groups: (1) Control group; (2) EA group; (3) CCl₄ group; (4) EA + CCl₄ group. In brain tissue, tumor necrosis factor‐α (TNF‐α), nuclear factor kappa b (NF‐kB), cyclooxygenase‐2 (COX‐2), nuclear erythroid related factor 2 (Nrf‐2), cysteine‐aspartic acid protease (caspase‐3), VEGF (vascular endothelial growth factor) and B‐cell lymphoma‐2 (bcl‐2) protein expression levels were analyzed by western blotting. MDA (malondialdehyde), catalase enzyme activity (CAT) and glutathione (GSH) analysis were determined by spectrophotometer. In our findings, EA ameliorated Nrf‐2 and caspase‐3 protein expression levels, GSH and catalase activities, NF‐kB, TNF‐α, VEGF, Bcl‐2, COX‐2 protein expression levels and MDA levels in CCl₄ intoxicated rats. These results suggest that EA demonstrated the neuroprotective effect on CCl₄‐induced brain damage in rats.

中文翻译：

---

鞣花酸通过调节Nrf-2，NF-kB和细胞凋亡途径对大鼠脑损伤的预防作用。

这项研究的目的是研究鞣花酸（EA）对CCl ₄诱导的大鼠脑损伤的神经保护作用。在这项研究中，将大鼠分为四组。分组：（1）对照组；（2）EA组；（3）CCl ₄组；（4）EA + CCl ₄组。在脑组织中，肿瘤坏死因子-α（TNF-α），核因子κb（NF-kB），环氧合酶-2（COX-2），核红系相关因子2（Nrf-2），半胱氨酸-天冬氨酸蛋白酶免疫印迹分析了caspase-3，VEGF（血管内皮生长因子）和B细胞淋巴瘤2（bcl-2）蛋白的表达水平。用分光光度计测定MDA（丙二醛），过氧化氢酶活性（CAT）和谷胱甘肽（GSH）。在我们的发现中，EA改善了CCl ₄中毒大鼠的Nrf-2和caspase-3蛋白表达水平，GSH和过氧化氢酶活性，NF-kB，TNF-α，VEGF，Bcl-2，COX-2蛋白表达水平和MDA水平。。这些结果表明，EA证明了对CCl ₄诱导的大鼠脑损伤的神经保护作用。