Closed-chamber methodology is widely used for the estimation of greenhouse gas (GHG) emissions in agricultural systems. The volume displaced by plants inside chambers influences GHG flux estimation, although generally it is not discounted from chamber headspace in the calculation. A novel image analysis-based procedure is proposed to estimate plant volume and to assess its impact on nitrous oxide (N2 O) flux estimations in a wheat (Triticum aestivum L. 'Rimbaud') crop. A maximum of 2.2% of the 13-L chambers was displaced by plants, leading to a systematic 0.9% overestimation in cumulative N2 O emissions if plant volume was not considered. Thus, plant canopy volume should be taken into account for improving the accuracy of emissions.

中文翻译：

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一氧化二氮排放研究中估算植物体积的简单方法

密室方法广泛用于估算农业系统中的温室气体 (GHG) 排放量。室内植物置换的体积影响温室气体通量估计，尽管在计算中通常不会从室内顶部空间中扣除。提出了一种基于图像分析的新程序来估计植物体积并评估其对小麦（Triticum aestivum L. 'Rimbaud'）作物中一氧化二氮 (N2 O) 通量估计的影响。最多 2.2% 的 13-L 室被植物取代，如果不考虑植物体积，则会导致系统高估累积 N2O 排放 0.9%。因此，应考虑植物冠层体积以提高排放的准确性。