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Novel lactone-layered double hydroxide ionomer powders for bone tissue repair.
Journal of Biomedical Materials Research Part B: Applied Biomaterials ( IF 3.4 ) Pub Date : 2020-04-10 , DOI: 10.1002/jbm.b.34614
Tianhao Zhou 1 , Edward D McCarthy 2 , Constantinos Soutis 1 , Sarah H Cartmell 1
Affiliation  

This article describes the use of a novel lactone‐layered double hydroxide polymer network (PN), derived from a poly(lactide‐co‐caprolactone) copolymer, as a controlled ion‐release agent for artificial bone tissue regeneration. The osteogenic cell culture Saos‐2 is used as a test culture to investigate the PN's performance as an extracellular ion‐release agent. The compelling performance of this PN is demonstrated in both growth and osteogenic media compared with a control of cells grown on tissue culture plastic (TCP) without PN. Firstly, the PNs released concentration of magnesium ions over time ranging from 10 to 60 mM after 24 hr, depending on the PN sample. After incubation of Saos‐2 with the PN, while no difference was seen in cell number, there was significant upregulation of bone‐related gene expression at 14 days—~5fold increase in Bone Morphogenetic Protein 2, ~3fold increase in osteopontin and ~2fold increase in collagen Type I. In addition, normalized alkaline phosphatase activity was seen to significantly increase by ~2fold with PN presence. A ~4fold increase in collagen Type I protein expression (via Gomori Trichrome Stain) was observed with PN presence. In addition, a ~4fold increase in phosphate deposits (as seen with Von Kossa staining analysis) was seen with PN presence. It is found that this novel PN material has a significant potential for bone tissue regeneration.

中文翻译:

用于骨组织修复的新型内酯层状双氢氧化物离聚物粉末。

本文描述了使用源自聚(丙交酯-共-己内酯)共聚物的新型内酯层状双氢氧化物聚合物网络(PN)作为人工骨组织再生的受控离子释放剂。成骨细胞培养物 Saos-2 用作测试培养物,以研究 PN 作为细胞外离子释放剂的性能。与在没有 PN 的组织培养塑料 (TCP) 上生长的细胞对照相比,这种 PN 的引人注目的性能在生长和成骨培养基中都得到了证明。首先,根据 PN 样品,PN 会在 24 小时后随时间释放 10 至 60 mM 的镁离子浓度。Saos-2 与 PN 孵育后,虽然细胞数量没有差异,14 天骨相关基因表达显着上调——骨形态发生蛋白 2 增加约 5 倍,骨桥蛋白增加约 3 倍,I 型胶原增加约 2 倍。此外,正常化碱性磷酸酶活性显着增加约 2 倍的 PN 存在。在 PN 存在的情况下,观察到 I 型胶原蛋白表达(通过 Gomori 三色染色)增加了约 4 倍。此外,在 PN 存在的情况下,磷酸盐沉积物增加了约 4 倍(如 Von Kossa 染色分析所示)。发现这种新型 PN 材料具有显着的骨组织再生潜力。发现标准化碱性磷酸酶活性随着 PN 的存在显着增加约 2 倍。在 PN 存在的情况下,观察到 I 型胶原蛋白表达(通过 Gomori 三色染色)增加了约 4 倍。此外,在 PN 存在的情况下,磷酸盐沉积物增加了约 4 倍(如 Von Kossa 染色分析所示)。发现这种新型 PN 材料具有显着的骨组织再生潜力。发现标准化碱性磷酸酶活性随着 PN 的存在显着增加约 2 倍。在 PN 存在的情况下,观察到 I 型胶原蛋白表达(通过 Gomori 三色染色)增加了约 4 倍。此外,在 PN 存在的情况下,磷酸盐沉积物增加了约 4 倍(如 Von Kossa 染色分析所示)。发现这种新型 PN 材料具有显着的骨组织再生潜力。
更新日期:2020-04-10
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