Journal of CO2 Utilization ( IF 7.7 ) Pub Date : 2020-04-11 , DOI: 10.1016/j.jcou.2020.101172 Hyukjoon Moon , Suhyeok Kim , Byung Hoon Jo , Hyung Joon Cha
Carbonic anhydrase (CA), an enzyme that catalyzes the hydration of CO2, has been considered an attractive green catalyst for bioinspired CO2 capture and utilization. The construction and utilization of a CO2-capturing whole-cell biocatalyst offers potential economic viability for industrial applications. The recently engineered whole-cell biocatalyst with periplasmic CA from Hydrogenovibrio marinus showed superior activity and stability over other systems. On the other hand, it was prone to enzyme leakage and cell lysis, necessitating whole-cell immobilization. Herein, the periplasmic whole-cell catalyst was entrapped in polyurethane foam by a facile one-step copolymerization method. The functional immobilization of cells was achieved with an immobilization efficiency of 3.4 %, which was 16-fold higher than that for free enzymes. The catalytic activity increased linearly depending on the cell density and the number of cut pieces of the immobilized catalyst. Notably, the reusability of the immobilized whole-cell catalyst was excellent, showing no apparent decrease in activity after nine reuses. Unexpectedly, the entrapment of cells in polyurethane foam was not effective for the thermal stabilization of enzymes presumably due to the lack of a direct covalent linkage of enzyme to the polymeric matrix. The rate of CO2 capture in the designed reactor was accelerated by 80 % using the immobilized biocatalyst. We expect that the immobilized whole-cell biocatalyst can be used as an efficient and economic biocatalyst for practical CO2 capture and utilization, and the performance of the system would be greatly improved by optimizing various parameters in the construction of the catalyst.
中文翻译:
用周质碳酸酐酶固定基因工程全细胞生物催化剂在聚氨酯泡沫中用于酶促CO 2的捕获和利用
碳酸酐酶(CA)是一种催化CO 2水合的酶,已被认为是一种有吸引力的绿色催化剂,可用于生物启发性的CO 2捕集和利用。捕获CO 2的全细胞生物催化剂的构建和利用为工业应用提供了潜在的经济可行性。最近设计的全细胞生物催化剂,具有来自海藻Hydrogenovibrio marinus的周质CA表现出优于其他系统的活性和稳定性。另一方面,它易于酶泄漏和细胞裂解,因此需要全细胞固定化。在此,通过简便的一步共聚法将周质全孔催化剂截留在聚氨酯泡沫中。细胞的功能性固定以3.4%的固定效率实现,比游离酶的固定效率高16倍。催化活性取决于细胞密度和固定化催化剂的切块数目而线性增加。值得注意的是,固定化全细胞催化剂的可重复使用性极好,经过九次重复使用后,活性没有明显降低。不料,推测,由于缺乏酶与聚合物基体的直接共价键,聚氨酯泡沫中的孔截留对酶的热稳定没有效果。CO率使用固定化的生物催化剂,可将设计好的反应器中的2捕获速度提高80%。我们期望固定化的全细胞生物催化剂可以用作有效和经济地用于实际CO 2捕集和利用的生物催化剂,并且通过优化催化剂的结构中的各种参数,可以大大提高系统的性能。