Cohesin is a conserved, ring-shaped protein complex that topologically entraps DNA. This ability makes this member of the structural maintenance of chromosomes (SMC) complex family a central hub of chromosome dynamics regulation. Besides its essential role in sister chromatid cohesion, cohesin shapes the interphase chromatin domain architecture and plays important roles in transcriptional regulation and DNA repair. Cohesin is loaded onto chromosomes at centromeres, at the promoters of highly expressed genes, as well as at DNA replication forks and sites of DNA damage. However, the features that determine these binding sites are still incompletely understood. We recently described a role of the budding yeast RSC chromatin remodeler in cohesin loading onto chromosomes. RSC has a dual function, both as a physical chromatin receptor of the Scc2/Scc4 cohesin loader complex, as well as by providing a nucleosome-free template for cohesin loading. Here, we show that the role of RSC in sister chromatid cohesion is conserved in fission yeast. We discuss what is known about the broader conservation of the contribution of chromatin remodelers to cohesin loading onto chromatin.

粘着蛋白是一种保守的环状蛋白质复合物，在拓扑结构上可以捕获DNA。这种能力使染色体结构维护（SMC）复杂家族的这一成员成为染色体动力学调节的中心枢纽。粘着蛋白除了在姐妹染色单体内聚力中起重要作用外，还可以塑造相间染色质结构域结构，并在转录调控和DNA修复中起重要作用。粘着蛋白在着丝粒，高表达基因的启动子，DNA复制叉和DNA损伤位点处被加载到染色体上。但是，确定这些结合位点的功能仍不完全了解。我们最近描述了发芽的酵母RSC染色质重塑剂在黏附素加载到染色体上的作用。RSC具有双重功能，既可以作为Scc2 / Scc4黏附素加载物复合物的物理染色质受体，也可以通过提供无核小体的黏附素加载模板来实现。在这里，我们表明在裂变酵母中RSC在姐妹染色单体内聚中的作用是保守的。我们讨论了有关染色质重塑剂对黏附在染色质上的黏附蛋白的贡献的更广泛保存的已知信息。