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Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry
Journal of Microbiology ( IF 3 ) Pub Date : 2019-11-23 , DOI: 10.1007/s12275-020-9297-y Yoontak Han , Eun-Jin Lee
Journal of Microbiology ( IF 3 ) Pub Date : 2019-11-23 , DOI: 10.1007/s12275-020-9297-y Yoontak Han , Eun-Jin Lee
The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.
中文翻译:
通过透射电子显微镜和免疫细胞化学检测沙门氏菌II型鞭毛的产生
细菌鞭毛是一种附肢结构,它为运动提供了一种手段,以在动荡的环境中促进生存。为了使细胞内病原体小肠鼠伤寒沙门氏菌能够在巨噬细胞中生存,鞭毛基因的表达必须受到严格调节,因此必须控制多个水平,包括DNA重组,转录,转录后,蛋白质合成以及宿主细胞内的组装。要了解鞭毛对宿主内沙门氏菌发病的贡献,通过显微镜检测巨噬细胞内鞭毛的产生至关重要。在本文中,我们描述了两种在体外和体内通过显微镜检测细菌鞭毛的方法 感染模型。
更新日期:2019-11-23
中文翻译:
通过透射电子显微镜和免疫细胞化学检测沙门氏菌II型鞭毛的产生
细菌鞭毛是一种附肢结构,它为运动提供了一种手段,以在动荡的环境中促进生存。为了使细胞内病原体小肠鼠伤寒沙门氏菌能够在巨噬细胞中生存,鞭毛基因的表达必须受到严格调节,因此必须控制多个水平,包括DNA重组,转录,转录后,蛋白质合成以及宿主细胞内的组装。要了解鞭毛对宿主内沙门氏菌发病的贡献,通过显微镜检测巨噬细胞内鞭毛的产生至关重要。在本文中,我们描述了两种在体外和体内通过显微镜检测细菌鞭毛的方法 感染模型。