The widespread use of the organochlorine insecticide lindane in the world has caused serious environmental problems. The main purpose of this paper is to investigate the potency of several Phlebia species of white rot fungi to degrade, transform and mineralize lindane, and to provide the feasibility of using white rot fungi for bioremediation at contaminated sites. Based on tolerance experiment results, Phlebia brevispora and Phlebia lindtneri had the highest tolerance to lindane and were screened by degradation tests. After 25 days of incubation, P. brevispora and P. lindtneri degraded 87.2 and 73.3% of lindane in low nitrogen medium and 75.8 and 64.9% of lindane in high nitrogen medium, respectively. Several unreported hydroxylation metabolites, including monohydroxylated, dehydroxylated, and trihydroxylated products, were detected and identified by GC/MS as metabolites of lindane. More than 10% of [¹⁴C] lindane was mineralized to ¹⁴CO₂ by two fungi after 60 days of incubation, and the mineralization was slightly promoted by the addition of glucose. Additionally, the degradation of lindane and the formation of metabolites were efficiently inhibited by piperonyl butoxide, demonstrating that cytochrome P450 enzymes are involved in the fungal transformation of lindane. The present study showed that P. brevispora and P. lindtneri were efficient degraders of lindane; hence, they can be applied in the bioremediation process of lindane-contaminated sites.

中文翻译：

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白腐真菌的侧柏种类对林丹有氧降解，转化和矿化的效力。

在世界范围内有机氯杀虫剂林丹的广泛使用已引起严重的环境问题。本文的主要目的是研究几种Phlebia种类的白腐真菌降解，转化和矿化林丹的潜力，并提供在污染场所使用白腐真菌进行生物修复的可行性。根据耐受性实验结果，紫草和紫草对林丹的耐受性最高，并通过降解测试进行筛选。孵育25天后，P。brevispora和P. lindtneri在低氮培养基中分别降解了87.2％和73.3％的林丹，在高氮培养基中分别降解了75.8和64.9％的林丹。GC / MS检测并鉴定了几种未报告的羟基化代谢物，包括单羟基化，脱羟基和三羟基化产物，它们是林丹的代谢物。孵育60天后，两种真菌将超过10％的[ ¹⁴ C]林丹矿化为¹⁴ CO ₂，并且通过添加葡萄糖略微促进了矿化。此外，胡椒基丁醇有效抑制了林丹的降解和代谢产物的形成，表明细胞色素P450酶参与了林丹的真菌转化。本研究表明，短孢子虫和林氏疟原虫是林丹的有效降解剂。因此，它们可用于林丹污染部位的生物修复过程。