Subcutaneous (SAT) and visceral (VAT) adipose tissues have distinct metabolic phenotypes. We hypothesized that the extracellular matrix (ECM) regulates depot-specific differences in adipocyte metabolic function in murine obesity. VAT and SAT preadipocytes from lean or obese mice were subject to adipogenic differentiation in standard 2D culture on plastic tissue culture plates or in 3D culture in ECM, followed by metabolic profiling. Adipocytes from VAT relative to SAT manifested impaired insulin-stimulated glucose uptake and decreased adipogenic capacity. In 3D-ECM-adipocyte culture, ECM regulated adipocyte metabolism in a depot-specific manner, with SAT ECM rescuing defects in glucose uptake and adipogenic gene expression in VAT adipocytes, while VAT ECM impaired adipogenic gene expression in SAT adipocytes. These findings demonstrate that ECM-adipocyte crosstalk regulates depot-specific differences in adipocyte metabolic dysfunction in murine obesity.

中文翻译：

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小鼠肥胖中的仓库特异性脂肪细胞-细胞外基质代谢串扰。

皮下 (SAT) 和内脏 (VAT) 脂肪组织具有不同的代谢表型。我们假设细胞外基质 (ECM) 调节小鼠肥胖中脂肪细胞代谢功能的库特异性差异。来自瘦小鼠或肥胖小鼠的 VAT 和 SAT 前脂肪细胞在塑料组织培养板上的标准 2D 培养或 ECM 的 3D 培养中进行脂肪形成分化，然后进行代谢分析。来自 VAT 的脂肪细胞相对于 SAT 表现出胰岛素刺激的葡萄糖摄取受损和脂肪形成能力降低。在 3D-ECM 脂肪细胞培养中，ECM 以库特异性方式调节脂肪细胞代谢，SAT ECM 挽救 VAT 脂肪细胞中葡萄糖摄取和脂肪形成基因表达的缺陷，而 VAT ECM 损害 SAT 脂肪细胞中的脂肪形成基因表达。