ABSTRACT

Chlorogenic acid is a well-known nutraceutical, but it is extensively metabolized by the body. More valuable information can be obtained from its metabolites. Dihydrocaffeic acid is a metabolite of chlorogenic acid and has shown antioxidant, cardioprotective, and neuroprotective effects; however, information about its anticancer activity is very scarce. Therefore, the main objective of this study was to determine the anticancer potential of dihydrocaffeic acid. The cancer cell lines used were MCF-7, Hep-G2, PC-3, and HCT-116, while HDFa was used as healthy cells. The cytotoxic concentrations to kill 50%, 75%, and 90% of the cells (CC₅₀, CC₇₅, CC₉₀) were determined using the MTS assay. Dihydrocaffeic acid was significantly more cytotoxic for most cancer cell lines, including MCF-7, PC-3, and HCT-116, compared with HDFa; however, Hep-G2 was significantly more resistant than HDFa. Dihydrocaffeic acid is a potential candidate for cancer prevention and treatment. The mechanism of action remains to be elucidated.

摘要

绿原酸是众所周知的保健食品，但它会被人体广泛代谢。可以从其代谢物中获得更多有价值的信息。二氢咖啡酸是绿原酸的代谢产物，具有抗氧化，保护心脏和保护神经的作用；但是，有关其抗癌活性的信息非常少。因此，这项研究的主要目的是确定二氢咖啡酸的抗癌潜力。使用的癌细胞系为MCF-7，Hep-G2，PC-3和HCT-116，而HDFa被用作健康细胞。杀死50％，75％和90％的细胞的细胞毒性浓度（CC ₅₀，CC ₇₅，CC ₉₀使用MTS分析确定）。与HDFa相比，二氢咖啡酸对包括MCF-7，PC-3和HCT-116在内的大多数癌细胞系的细胞毒性明显更高。但是，Hep-G2的抗药性明显高于HDFa。二氢咖啡酸是预防和治疗癌症的潜在候选人。作用机理尚待阐明。