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Validation and identification of reference genes in Chinese hamster ovary cells for Fc-fusion protein production.
Experimental Biology and Medicine ( IF 3.2 ) Pub Date : 2020-03-26 , DOI: 10.1177/1535370220914058
Xiaonan Ma 1 , Ling Zhang 1 , Luming Zhang 1 , Chenglong Wang 1 , Xiaorui Guo 1 , Yu Yang 1 , Lin Wang 1 , Xiangru Li 1 , Ningning Ma 1
Affiliation  

In order to reveal potential genotype-phenotype relationship, RT-qPCR reactions are frequently applied which require validated and reliable reference genes. With the investigation on long-term passage and fed-batch cultivation of CHO cells producing an Fc-fusion protein, four new reference genes-Akr1a1, Gpx1, Aprt, and Rps16, were identified from 20 candidates with the aid of geNorm, NormFinder, BestKeeper, and ΔCt programs and methods. This article provided more verified options in reference gene selection in related research on CHO cells.
更新日期:2020-04-20
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