The present study reports the effects of a high-fat (HF) diet of over 8 weeks on the Fe status of growing rats. Tissue Fe levels were analysed by atomic absorption spectrophotometry, and whole-body adiposity was measured by dual-energy X-ray absorptiometry. Histopathology and morphometry of adipose tissue were performed. Liver homogenates were used for measuring ferroportin-1 protein levels by immunoblotting, and transcript levels were used for Fe genes measured by real-time PCR. Tissue Fe pools were fit to a compartmental biokinetic model in which Fe was assessed using fourteen compartments and twenty-seven transfer constants (kj,i from tissue ‘i’ to tissue ‘j’) adapted from the International Commission on Radiological Protection (ICRP) 69. Ten kj,i were calculated from the experimental data using non-linear regression, and seventeen were estimated by allometry according to the formula ${k_{i,j}} = a \times {M^b}$. Validation of the model was carried out by comparing predicted and analysed Fe pool sizes in erythrocytes, the liver and the spleen. Body adiposity was negatively associated with serum Fe levels and positively associated with liver Fe stores. An inferred increase in Fe transfer from bone marrow to the liver paralleled higher hepatic Fe concentrations and ferritin heavy-chain mRNA levels in the HF diet-fed animals, suggesting that liver Fe accumulation occurred at least in part due to a favoured liver erythrocyte uptake. If this feeding condition was to be prolonged, impaired Fe decompartmentalisation may occur, ultimately resulting in dysmetabolic Fe overload.

中文翻译：

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给生长中的老鼠喂食高脂肪饮食会增加肥胖导致铁的去室化

本研究报告了超过 8 周的高脂肪 (HF) 饮食对生长大鼠铁状态的影响。通过原子吸收分光光度法分析组织Fe水平，并通过双能X射线吸收法测量全身肥胖。进行脂肪组织的组织病理学和形态计量学。肝匀浆用于通过免疫印迹测量 ferroportin-1 蛋白水平，转录水平用于通过实时 PCR 测量的 Fe 基因。组织 Fe 池适合隔室生物动力学模型，其中使用 14 个隔室和 27 个传递常数评估 Fe（ķj,我从组织‘i’到组织‘j’)改编自国际放射防护委员会 (ICRP) 69. 十ķj,我使用非线性回归从实验数据计算，并根据公式通过异速生长法估计十七${k_{i,j}} = a \times {M^b}$. 通过比较预测和分析的红细胞、肝脏和脾脏中的铁池大小来验证模型。身体肥胖与血清铁水平呈负相关，与肝脏铁储存呈正相关。在 HF 饮食喂养的动物中，从骨髓到肝脏的 Fe 转移的推断增加与较高的肝脏 Fe 浓度和铁蛋白重链 mRNA 水平平行，这表明肝脏 Fe 积累的发生至少部分是由于有利于肝脏红细胞摄取。如果要延长这种喂养条件，可能会发生受损的铁去隔室化，最终导致代谢异常的铁过载。