RNA modifications play important roles in RNA structures and regulation of gene expression and translation. We report the first RNA modification on the phosphate, the RNA phosphorothioate (PS) modification, discovered in both prokaryotes and eukaryotes. The PS modification is also first reported on nucleic acids of eukaryotes. The GpsG modification exists in the Rp configuration and was quantified with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). By knocking out the DndA gene in E. coli, we show the Dnd clusters that regulate DNA PS modification may also play roles in RNA PS modification. We also show that the GpsG modification locates on rRNA in E. coli, L. lactis, and HeLa cells, and it is not detected in rRNA-depleted total RNAs from these cells.

中文翻译：

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原核生物和真核生物中的RNA硫代磷酸酯修饰。

RNA修饰在RNA结构以及基因表达和翻译的调节中起重要作用。我们报道了在原核生物和真核生物中发现的磷酸盐上的第一个RNA修饰，RNA硫代磷酸酯（PS）修饰。PS修饰也首先报道在真核生物的核酸上。GpsG修饰以R p构型存在，并通过液相色谱和串联质谱（LC-MS / MS）进行定量。通过敲除大肠杆菌中的Dnd A基因，我们显示出调节DNA PS修饰的Dnd簇也可能在RNA PS修饰中起作用。我们还表明，在rRNA基因的GPSG修改所处的大肠杆菌，乳酸乳球菌和HeLa细胞，并且在这些细胞的rRNA耗尽的总RNA中未检测到。