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Organic secretion in ductal cells of the sublingual salivary gland of ferret: Histochemical observations.
Acta Histochemica ( IF 2.5 ) Pub Date : 2020-04-10 , DOI: 10.1016/j.acthis.2020.151540
Asterios Triantafyllou 1 , David Fletcher 2
Affiliation  

The presence of organic secretion in ductal cells of the sublingual salivary gland of ferret has been questioned, which prompted the present investigation. Paraffin or cryostat sections from aldehyde fixed or quenched sublingual glands of this species were tested for some amino acid residues, mucosubstances, oxidative and hydrolytic enzymes, and lectins. The glands showed inconspicuous ducts of simple appearances on routine histology. The histochemical procedures, however, revealed a granulated substance in the apical (periluminal) region of ductal cells, which contained tryptophan, disulphides, neutral mucosubstances, αFuc and GalNAc, and showed chloroacetate esterase activity. Occurrence of the substance varied between different ducts of the same gland and/or cells of the same duct. The ductal cells also showed diffuse peroxidase and acid phosphatase, and Golgi-like thiamine pyrophosphatase activities. Acetylcholinesterase-positive nerve fibres embraced the ducts. The results support a particular localisation of protein-bound amino acid residues and enzymatic catalytic activities indicative of organic secretion, possibly tissue kallikrein, in sublingual ductal cells of ferret.

中文翻译:

雪貂舌下唾液腺导管细胞的有机分泌:组织化学观察。

对雪貂舌下唾液腺导管细胞中有机分泌物的存在提出了质疑,这促使了本研究。测试了该物种的醛固定或淬灭舌下腺的石蜡或低温恒温器切片的一些氨基酸残基,粘膜物质,氧化酶和水解酶以及凝集素。腺体在常规组织学上显示出不明显的简单外观的导管。但是,组织化学程序显示导管细胞的顶端(管腔周围)有颗粒状物质,其中含有色氨酸,二硫化物,中性粘膜物质,αFuc和GalNAc,并显示出氯乙酸酯酶的活性。在相同腺体的不同导管之间和/或在相同导管的细胞之间,物质的存在发生变化。导管细胞还表现出弥散的过氧化物酶和酸性磷酸酶,以及高尔基样的硫胺素焦磷酸酶活性。乙酰胆碱酯酶阳性的神经纤维包裹着导管。结果支持在雪貂舌下导管细胞中蛋白质结合的氨基酸残基的特定定位和指示有机分泌的酶催化活性,可能是组织激肽释放酶。
更新日期:2020-04-10
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