CD300a receptor is found on different CD8+ T cell subsets and its expression has been associated to a more cytotoxic molecular signature. CD300a has an important role in some viral infections and its expression levels are known to be modulated by human immunodeficiency virus (HIV)−1 infection on several cell types. The main objective of this work was to investigate CD300a expression and its regulation during HIV-1 specific CD8+ T cell responses. CD300a receptor expression was analysed by multiparametric flow cytometry on CD8+ T lymphocytes from HIV negative donors, naive HIV-1+ individuals and HIV-1+ subjects under suppressive combined antiretroviral therapy (cART). HIV-1 specific CD8+ T cell response was studied by stimulating cells with HIV-1 derived peptides or with a Gag HIV-1 peptide. Our results showed that HIV-1 specific CD8+ T cells expressing higher levels of CD300a were more polyfunctional showing an increased degranulation and cytokine production. Moreover, we observed an up-regulation of CD300a expression after Gag HIV-1 peptide stimulation. Finally, our results demonstrated an inverse correlation between CD300a expression on CD8+ T lymphocytes and HIV disease progression markers. In conclusion, CD300a expression is associated to a better and more polyfunctional HIV-1 specific CD8+ T cell response.

CD300a受体存在于不同的CD8 + T细胞亚群中，其表达与更多的细胞毒性分子标记有关。CD300a在某些病毒感染中起着重要作用，并且已知其表达水平受人类免疫缺陷病毒（HIV）-1感染几种细胞类型的调节。这项工作的主要目的是研究CD300a的表达及其在HIV-1特异性CD8 + T细胞应答中的调控。通过多参数流式细胞术在抑制性联合抗逆转录病毒疗法（cART）下，对来自HIV阴性供体，未感染HIV-1 +个体和HIV-1 +受试者的CD8 + T淋巴细胞进行多参数流式细胞术分析。通过用HIV-1衍生肽或Gag HIV-1肽刺激细胞来研究HIV-1特异性CD8 + T细胞应答。我们的结果表明，表达更高水平的CD300a的HIV-1特异性CD8 + T细胞具有更高的多功能性，显示出更高的脱颗粒和细胞因子产生。此外，我们观察到Gag HIV-1肽刺激后CD300a表达的上调。最后，我们的结果表明CD8 + T淋巴细胞上CD300a表达与HIV疾病进展标志物呈负相关。总之，CD300a表达与更好且更具多功能性的HIV-1特异性CD8 + T细胞反应相关。我们的结果证明了CD8 + T淋巴细胞上CD300a表达与HIV疾病进展标志物呈负相关。总之，CD300a表达与更好且更具多功能性的HIV-1特异性CD8 + T细胞反应相关。我们的结果证明CD8 + T淋巴细胞上CD300a表达与HIV疾病进展标志物呈负相关。总之，CD300a表达与更好和更具多功能性的HIV-1特异性CD8 + T细胞反应相关。