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Composition, Predicted Functions and Co-occurrence Networks of Rhizobacterial Communities Impacting Flowering Desert Events in the Atacama Desert, Chile.
Frontiers in Microbiology ( IF 5.2 ) Pub Date : 2020-04-08 , DOI: 10.3389/fmicb.2020.00571
Marcia Astorga-Eló 1, 2 , Qian Zhang 3 , Giovanni Larama 2 , Alexandra Stoll 4 , Michael J Sadowsky 3, 5, 6 , Milko A Jorquera 2, 7
Affiliation  

Flowering desert (FD) events consist of the rapid flowering of a wide variety of native plants in the Atacama Desert of Chile, which is categorized as the driest desert in the world. While ephemeral plants are an integral part of the desert ecosystem, there is little knowledge on plant-microbe interactions that occur during FD events. Consequently, the overall goals of this present study were to investigate changes in the composition and potential functions of rhizobacterial community of Cistanthe longiscapa (Montiaceae) during the 2014 and 2015 FD events and determine the composition, potential functions, and co-occurrence networks of rhizobacterial community associated with the root zone of C. longiscapa during pre- (PF) and full-flowering (FF) phenological stages. Results of this study showed that the Proteobacteria and Actinobacteria were the dominant taxa in rhizosphere soils during the three FD events (2014, 2015, and 2017) examined. In general, greater microbial richness and diversity were observed in rhizosphere soils during the 2015-, compared with the 2014-FD event. Similarly, predicted functional analyses indicated that a larger number of sequences were assigned to information processing (e.g., ion channel, transporters and ribosome) and metabolism (e.g., lipids, nitrogen, and sulfur) during 2015 compared with 2014. Despite the lack of significant differences in diversity among PF and FF stages, the combined analysis of rhizobacterial community data, along with data concerning rhizosphere soil properties, evidenced differences among both phenological stages and suggested that sodium is a relevant abiotic factor shaping the rhizosphere. In general, no significant differences in predicted functions (most of them assigned to chemoheterotrophy, magnesium metabolisms, and fermentation) were observed among PF and FF. Co-occurrence analysis revealed the complex rhizobacterial interactions that occur in C. longiscapa during FD, highlighting to Kouleothrixaceae family as keystone taxa. Taken together this study shows that the composition and function of rhizobacteria vary among and during FD events, where some bacterial groups and their activity may influence the growth and flowering of native plants, and therefore, the ecology and trophic webs in Atacama Desert.

中文翻译:

影响智利阿塔卡马沙漠开花沙漠事件的根瘤菌群落的组成,预测功能和共现网络。

沙漠开花(FD)事件包括智利阿塔卡马沙漠中被称为世界上最干燥沙漠的各种本地植物的快速开花。尽管短暂植物是沙漠生态系统的组成部分,但对FD事件期间发生的植物与微生物相互作用的知识很少。因此,本研究的总体目标是调查2014年和2015年FD事件期间肉Ci蓉(Montiaceae)根瘤菌群落组成和潜在功能的变化,并确定根瘤菌的组成,潜在功能和共现网络在物候期(PF)和盛花期(FF)期间,与长枝假丝酵母根区相关的群落。这项研究的结果表明,在3个FD事件(2014年,2015年和2017年)调查期间,变形杆菌和放线菌是根际土壤中的主要分类单元。总体而言,与2014-FD事件相比,2015-2015年根际土壤中微生物的丰富度和多样性更高。同样,预测的功能分析表明,与2014年相比,2015年期间为信息处理(例如离子通道,转运蛋白和核糖体)和新陈代谢(例如脂质,氮和硫)分配了更多序列。 PF和FF阶段之间多样性的差异,根瘤菌群落数据的综合分析以及有关根际土壤特性的数据,证据表明这两个物候阶段之间存在差异,并表明钠是塑造根际的相关非生物因子。通常,PF和FF之间的预测功能(大部分归因于化学趋化,镁代谢和发酵)没有显着差异。共现分析揭示了FD期间在长毛锦葵中发生的复杂的根际细菌相互作用,这对古兰科家族是重要的分类单元。综上所述,这项研究表明,在FD事件中和FD事件期间,根瘤菌的组成和功能各不相同,其中一些细菌群体及其活性可能影响本地植物的生长和开花,从而影响阿塔卡马沙漠的生态和营养网。在PF和FF之间,未观察到预测功能的显着差异(大多数归因于化学趋化,镁代谢和发酵)。共现分析揭示了FD期间在长毛锦葵中发生的复杂的根际细菌相互作用,这对古兰科家族是重要的分类单元。综上所述,这项研究表明,在FD事件中和FD事件期间,根瘤菌的组成和功能各不相同,其中一些细菌群体及其活性可能影响本地植物的生长和开花,从而影响阿塔卡马沙漠的生态和营养网。在PF和FF之间,未观察到预测功能的显着差异(大多数归因于化学趋化,镁代谢和发酵)。共现分析揭示了FD期间在长毛锦葵中发生的复杂的根际细菌相互作用,这对古兰科家族是重要的分类单元。总之,这项研究表明,在FD事件中和FD事件期间,根瘤菌的组成和功能各不相同,其中一些细菌群体及其活性可能影响本地植物的生长和开花,进而影响阿塔卡马沙漠的生态和营养网。重点向古兰科家族作为重点分类单元。综上所述,这项研究表明,在FD事件中和FD事件期间,根瘤菌的组成和功能各不相同,其中一些细菌群体及其活性可能影响本地植物的生长和开花,从而影响阿塔卡马沙漠的生态和营养网。重点向古兰科家族作为重点分类单元。综上所述,这项研究表明,在FD事件中和FD事件期间,根瘤菌的组成和功能各不相同,其中一些细菌群体及其活性可能影响本地植物的生长和开花,从而影响阿塔卡马沙漠的生态和营养网。
更新日期:2020-04-08
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