当前位置: X-MOL 学术Plant Cell › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Arabidopsis JAZ Proteins Interact with and Suppress RHD6 Transcription Factor to Regulate Jasmonate-Stimulated Root Hair Development
The Plant Cell ( IF 11.6 ) Pub Date : 2020-04-01
Han, X., Zhang, M., Yang, M., Hu, Y.

Root hairs arise from trichoblasts and are crucial for plant anchorage, nutrient acquisition, and environmental interactions. The phytohormone jasmonate is known to regulate root hair development in Arabidopsis (Arabidopsis thaliana), but little is known about the molecular mechanism underlying jasmonate modulation in this process. Here, we show that the application of exogenous jasmonate significantly stimulated root hair elongation, but, on the contrary, blocking the perception or signaling of jasmonate resulted in defective root hairs. Jasmonate consistently elevated the expression levels of several crucial genes positively involved in root hair growth. Mechanistic investigation revealed that JASMONATE ZIM-DOMAIN (JAZ) proteins, critical repressors of jasmonate signaling, physically interacted with ROOT HAIR DEFECTIVE 6 (RHD6) and RHD6 LIKE1 (RSL1), two transcription factors that are essential for root hair development. JAZ proteins inhibited the transcriptional function of RHD6 and interfered with the interaction of RHD6 with RSL1. Genetic analysis indicated that jasmonate promoted root hair growth in a RHD6/RSL1-dependent manner. Moreover, overexpression of RHD6 largely rescued the root hair defects of JAZ-accumulating plants. Collectively, our study reveals a key signaling module in which JAZ repressors of the jasmonate pathway directly modulate RHD6 and RSL1 transcription factors to integrate jasmonate signaling and the root hair developmental process.



中文翻译:

拟南芥JAZ蛋白与RHD6转录因子相互作用并抑制RHD6转录因子调节茉莉酸酯刺激的根毛发育。

根毛来自单核细胞,对于植株固着,养分获取和环境相互作用至关重要。已知茉莉酸植物激素可调节拟南芥(Arabidopsis thaliana)的根毛发育),但对该过程中茉莉酸调节的潜在分子机制知之甚少。在这里,我们显示外源茉莉酸酯的应用显着刺激了根毛的伸长,但是相反,阻止茉莉酸酯的感知或信号传导导致了根毛的缺陷。茉莉酸酯持续提高积极参与根毛生长的几个关键基因的表达水平。机理研究表明,茉莉酸信号的关键阻遏物JASMONATE ZIM-DOMAIN(JAZ)蛋白与根毛发育缺陷6(RHD6)和RHD6 LIKE1(RSL1)物理相互作用,这两个转录因子对于根发的发育至关重要。JAZ蛋白抑制RHD6的转录功能,并干扰RHD6与RSL1的相互作用。遗传分析表明,茉莉酸酯以RHD6 / RSL1依赖性方式促进根毛生长。而且,过表达RHD6很大程度上挽救了JAZ积累植物的根发缺陷。总的来说,我们的研究揭示了一个关键的信号传导模块,其中茉莉酸途径的JAZ阻遏物直接调节RHD6和RSL1转录因子,以整合茉莉酸信号和根毛发育过程。

更新日期:2020-04-21
down
wechat
bug