INTRODUCTION Bovine female and male embryos differentially release metabolites with signalling effects to culture media. However, it is unknown if the embryo-maternal interface (EMI) metabolome is modified by embryonic sex. OBJECTIVE To analyse using a combination of 1H NMR and a co-culture of endometrial cells the EMI. RESULTS Twenty-six metabolites were identified and quantified in the EMI, nine metabolites reflected the sex of the embryo rather than their presence. CONCLUSIONS 1H NMR is sensitive enough to perform quantitative analysis of sex-induced differences in the EMI. These results may help to understand the embryo-maternal dialogue on the basis of embryonic sex.

中文翻译：

---

胚胎-母体界面处雌雄孵化前胚胎代谢产物的核磁共振分析。

引言牛的雌性和雄性胚胎以不同的方式释放代谢物，并向培养基发出信号。但是，尚不清楚胚胎-母体界面（EMI）代谢组是否通过胚胎性改变。目的结合1H NMR和子宫内膜细胞共培养物进行EMI分析。结果在EMI中鉴定并定量了26种代谢物，其中9种代谢物反映了胚胎的性别而不是它们的存在。结论1 H NMR足够灵敏，可以对EMI中性别引起的差异进行定量分析。这些结果可能有助于了解基于胚胎性别的胚胎与母亲的对话。