当前位置: X-MOL 学术Int. J. Biol. Macromol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Photophysical behavior of heme group: Unfolding of hemoglobin and myoglobin in the presence of Gemini surfactants of different molecular architectures.
International Journal of Biological Macromolecules ( IF 8.2 ) Pub Date : 2020-04-08 , DOI: 10.1016/j.ijbiomac.2020.04.008
Lavanya Tandon 1 , Pankaj Thakur 2 , Kultar Singh 3 , Poonam Khullar 4 , Gurinder Kaur Ahluwalia 5 , Mandeep Singh Bakshi 6
Affiliation  

Fluorescence studies were performed to determine the photophysical behavior of heme group in the presence of cationic Gemini surfactants of different architectures. Both hemoglobin and myoglobin were used to understand the heme group interactions with Gemini surfactants under the influence of temperature variation and were compared with homologous monomeric surfactants. The results were also supplemented from the size and zeta potential measurements of both proteins. Gemini surfactants showed marked effect on the unfolding behavior of hemoglobin that mainly contributed by the stronger hydrophobic interactions of double hydrocarbon chains as well as methylene spacer in the head group region with the hydrophobic domains of hemoglobin. Myoglobin with single polypeptide chain did not show similar unfolding behavior in the presence of Gemini surfactants rather it was readily solubilized in the surfactant solution and that too in the presence of monomeric surfactants rather than Gemini surfactants. The results highlighted the mechanistic aspects by which water soluble globular proteins interact with amphiphilic molecules of different functionalities and thus, helped to predict the interactions of both hemoglobin and myoglobin with the complex biological molecules possessing similar functionalities.

中文翻译:

血红素族的光物理行为:在不同分子结构的双子座表面活性剂存在下,血红蛋白和肌红蛋白的展开。

在存在不同结构的阳离子Gemini表面活性剂的情况下,进行荧光研究以确定血红素基团的光物理行为。血红蛋白和肌红蛋白均用于了解在温度变化的影响下与双子表面活性剂的血红素基团的相互作用,并与同源单体表面活性剂进行比较。两种蛋白质的大小和Zeta电位测量结果也得到了补充。双子表面活性剂对血红蛋白的展开行为表现出显着影响,这主要是由于双烃链以及头部基团区域中的亚甲基间隔基与血红蛋白的疏水域之间更强的疏水相互作用所致。在Gemini表面活性剂的存在下,具有单多肽链的肌红蛋白没有显示出相似的解折叠行为,而是易于溶解在表面活性剂溶液中,并且在单体表面活性剂而非Gemini表面活性剂的存在下也易于溶解。该结果突出了水溶性球蛋白与不同功能的两亲分子相互作用的机理,从而有助于预测血红蛋白和肌红蛋白与具有相似功能的复杂生物分子之间的相互作用。
更新日期:2020-04-08
down
wechat
bug