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Pioneer trees of Betula pendula at a red gypsum landfill harbour specific structure and composition of root-associated microbial communities.
Science of the Total Environment ( IF 9.8 ) Pub Date : 2020-04-08 , DOI: 10.1016/j.scitotenv.2020.138530
Vanessa Álvarez-López 1 , Cyril Zappelini 1 , Alexis Durand 1 , Michel Chalot 2
Affiliation  

The study of root-associated microbial communities is important to understand the natural processes involved in plant recolonisation at degraded areas. Root associated bacterial and fungal communities of woody species colonising a red gypsum landfill (a metal-enriched environment) were characterised through metabarcoding. Among trees naturally growing on the landfill, Betula pendula is the only tree species in the centre of the area, whereas companion tree species such as Populus nigra, P. tremula and Salix purpurea were present on the edges. The bacterial community was dominated by Proteobacteria (38%), Actinobacteria (35%) and Bacteroidetes (20%) and the most abundant bacterial OTU belonged to the family Streptomycetaceae. The fungal community was dominated by Ascomycota (60%) and Basidiomycota (30%) and the most abundant family was Pyronemataceae. Analysis of similarities, heatmap and hierarchical cluster analysis showed that B. pendula grown in the centre of the landfill harboured a specific microbial community, which was unique and different, not only from other tree species (Populus or Salix spp.), but also from other B. pendula growing at the edges. Our findings on relevant indicator OTUs associated to the birches located in the centre of the landfill (such as Otu00716 Catellatospora sp. (family Micromonosporaceae, phylum Actinobacteria) or Otu4_35502 Russula sp. (family Russulaceae, phylum Basidiomycota)) may have important implications for the successful revegetation of these harsh environments using microbial-based phytostabilisation approaches.

中文翻译:

红石膏掩埋处的桦木先锋树具有与根相关的微生物群落的特定结构和组成。

根相关微生物群落的研究对于了解退化地区植物重新定殖所涉及的自然过程非常重要。通过metabarcoding表征了在红色石膏垃圾填埋场(富含金属的环境)中定居的木质物种的根系相关细菌和真菌群落。在垃圾填埋场自然生长的树木中,Betula pendula是该区域中心唯一的树木,而边缘的树木则是黑杨,P。tremula和Salix purpurea等伴生树种。细菌群落以变形杆菌(38%),放线菌(35%)和拟杆菌(20%)为主,最丰富的细菌OTU属于链霉菌科。真菌群落主要为子囊菌(60%)和担子菌(30%),而家族最多的是火棘科。对相似性,热图和层次聚类分析的分析表明,在垃圾填埋场中心生长的B. pendula具有特定的微生物群落,这种微生物群落不仅与其他树种(杨属或柳属)不同,而且与众不同。其他B. pendula生长在边缘。我们对与位于垃圾填埋场中心的桦树相关的相关指标OTU的发现(例如Otu00716 Catellatospora sp。(微单孢菌科,Actinobacteria属)或Otu4_35502 Russula sp。(Russulaceae,Basidiomycota属)可能具有重要意义。使用基于微生物的植物稳定方法成功地在这些恶劣的环境中恢复植被。对相似性,热图和层次聚类分析的分析表明,在垃圾填埋场中心生长的B. pendula具有特定的微生物群落,这种微生物群落不仅与其他树种(杨属或柳属)不同,而且与众不同。其他B. pendula生长在边缘。我们对与位于垃圾填埋场中心的桦树相关的相关指标OTU的发现(例如Otu00716 Catellatospora sp。(微单孢菌科,Actinobacteria属)或Otu4_35502 Russula sp。(Russulaceae,Basidiomycota属)可能具有重要意义。使用基于微生物的植物稳定方法成功地在这些恶劣的环境中恢复植被。对相似性,热图和层次聚类分析的分析表明,在垃圾填埋场中心生长的B. pendula具有特定的微生物群落,这种微生物群落不仅与其他树种(杨属或柳属)不同,而且与众不同。其他B. pendula生长在边缘。我们对与位于垃圾填埋场中心的桦树相关的相关指标OTU的发现(例如Otu00716 Catellatospora sp。(微单孢菌科,Actinobacteria属)或Otu4_35502 Russula sp。(Russulaceae,Basidiomycota属)可能具有重要意义。使用基于微生物的植物稳定方法成功地在这些恶劣的环境中恢复植被。在垃圾填埋场中部生长的小飞虱具有独特的微生物群落,不仅与其他树种(杨属或柳树属),而且与边缘生长的其他B. Pendula独特且不同。我们对与位于垃圾填埋场中心的桦树相关的相关指标OTU的发现(例如Otu00716 Catellatospora sp。(微单孢菌科,Actinobacteria属)或Otu4_35502 Russula sp。(Russulaceae,Basidiomycota属)有重要意义。使用基于微生物的植物稳定方法成功地在这些恶劣的环境中恢复植被。在垃圾填埋场中部生长的小飞虱具有独特的微生物群落,不仅与其他树种(杨属或柳树属),而且与边缘生长的其他B. Pendula独特且不同。我们对与位于垃圾填埋场中心的桦树相关的相关指标OTU的发现(例如Otu00716 Catellatospora sp。(微单孢菌科,Actinobacteria属)或Otu4_35502 Russula sp。(Russulaceae,Basidiomycota属)可能具有重要意义。使用基于微生物的植物稳定方法成功地在这些恶劣的环境中恢复植被。
更新日期:2020-04-08
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