Heme oxygenase-1(HO-1) is a stress-inducible enzyme that mediates antioxidative and cytoprotective effects to maintain cellular redox homeostasis. In the present study, the full sequence of HO-1 was cloned from golden pompano, Trachinotus ovatus, by RT-PCR and RACE-PCR. The full cDNA sequence of HO-1 was 1349 bp in length which comprised of a 726 bp open reading frame (ORF) preceded by 262 bp 5′-untranslated region (UTR), and followed a 360 bp 3′UTR, encoding 241 amino acid residues. Phylogenetic analysis revealed that HO-1 showed highest similarity to that of Takifugu rubripes. Tissue distribution analysis showed that the expression level of HO-1 was relatively high in heart, liver and spleen. A trial was conducted to investigate the response of Nrf2/HO-1 signaling pathway to oxidative stress induced by copper. The results showed that mRNA expression of NF-E2-related nuclear factor2 (Nrf2), Kelch-like-ECH-associated protein1 (keap1), superoxide dismutase (SOD), catalase (CAT), HO-1, NAD(P)H quinone oxidoreductase 1 (NQO1) and GSH-PX all significantly increased in copper treated group than that in the control group. This work provides new insight into the molecular mechanism underlying the Nrf2/HO-1 pathway in oxidative response in T. ovatus.

血红素加氧酶-1（HO-1）是一种应激诱导型酶，介导抗氧化和细胞保护作用，以维持细胞氧化还原稳态。在本研究中，通过RT-PCR和RACE-PCR从金po，卵形曲霉中克隆了HO-1的完整序列。HO-1的完整cDNA序列长度为1349 bp，由一个726 bp的开放阅读框（ORF）加上262 bp的5'-非翻译区（UTR）和360 bp的3'UTR组成，编码241个氨基酸酸残基。系统发育分析表明，HO-1与T柳的相似性最高。。组织分布分析表明，HO-1在心脏，肝脏和脾脏中的表达水平较高。进行了一项试验，以研究Nrf2 / HO-1信号通路对铜诱导的氧化应激的响应。结果表明，NF-E2相关核因子2（Nrf2），Kelch样ECH相关蛋白1（keap1），超氧化物歧化酶（SOD），过氧化氢酶（CAT），HO-1，NAD（P）H的mRNA表达铜处理组的醌氧化还原酶1（NQO1）和GSH-PX均明显高于对照组。这项工作提供了新的见解的分子机制的Nrf2 / HO-1途径的氧化卵线虫的反应。